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New Iranian and Australian peach latent mosaic viroid variants and evidence for rapid sequence evolution
- Source :
- Archives of virology. 157(2)
- Publication Year :
- 2011
-
Abstract
- Peach latent mosaic viroid isolates from peach and plum in Iran have been compared with an Australian isolate from nectarine. Thirteen sequence variants 336-338 nt in size were obtained. All variants clustered phylogenetically with variants reported from several hosts and countries. A total nucleic acid extract, a slightly longer than full-length RT-PCR amplicon, and a recombinant plasmid clone from the Australian isolate were all infectious to, and symptomatic in, mechanically inoculated peach seedlings. The infectious clone generated two progeny viroid molecules, which each showed 10 different mutations compared with the parent clone inoculated 30 days previously.
- Subjects :
- Genetics
clone (Java method)
biology
Base Sequence
Viroid
Molecular Sequence Data
Australia
Genetic Variation
General Medicine
Amplicon
Iran
biology.organism_classification
Virology
Viroids
Avsunviroidae
Evolution, Molecular
Prunus
Plant virus
Genetic variation
Nucleic Acid Conformation
Peach latent mosaic viroid
Phylogeny
Plant Diseases
Subjects
Details
- ISSN :
- 14328798
- Volume :
- 157
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Archives of virology
- Accession number :
- edsair.doi.dedup.....6b700380db05ecea4f00de670ad116c2