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On-chip immunofluorescence analysis of single cervical cells using an electroactive microwell array with barrier for cervical screening
- Source :
- Biomicrofluidics
- Publication Year :
- 2019
- Publisher :
- AIP Publishing, 2019.
-
Abstract
- Several specific tests for cervical screening have been developed recently, including p16/Ki67 dual immunostaining for diagnosing high-risk human papillomavirus positive squamous intraepithelial lesion in the cervix. However, manual screening of cells in an entire glass slide is currently a standard clinical procedure for quantification and interpretation of immunocytochemical features of the cells. Here, we developed a microfluidic device containing an electroactive microwell array with barriers (EMAB) for highly efficient single-cell trapping followed by on-chip immunofluorescence analysis with minimum loss of the sample. EMAB utilizes patterned electrodes at the bottom of cell-sized microwells to trap single cells using dielectrophoresis (DEP) and cell-holding structures behind the microwells to stabilize the position of trapped cells even without DEP. Using the device, we evaluated the performance of p16/Ki67 dual immunostaining of HeLa cells on the chip. The device shows 98% cell-trapping efficiency as well as 92% cell-holding efficiency against the fixed HeLa cells, and we successfully demonstrated high-efficiency on-chip immunofluorescence analysis with minimal loss of sample. p16/Ki67 dual immunostaining using EMAB may be useful for complementary tests for cervical screening in confirming the histopathological diagnosis.
- Subjects :
- Microfluidics
Biomedical Engineering
02 engineering and technology
Immunofluorescence
01 natural sciences
HeLa
Colloid and Surface Chemistry
medicine
General Materials Science
Cervix
Fluid Flow and Transfer Processes
Cervical screening
medicine.diagnostic_test
biology
Chemistry
010401 analytical chemistry
Dielectrophoresis
021001 nanoscience & nanotechnology
Condensed Matter Physics
medicine.disease
biology.organism_classification
0104 chemical sciences
Squamous intraepithelial lesion
medicine.anatomical_structure
0210 nano-technology
Immunostaining
Regular Articles
Biomedical engineering
Subjects
Details
- ISSN :
- 19321058
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Biomicrofluidics
- Accession number :
- edsair.doi.dedup.....6b6a186b183e31b0a5cce554fa649a32
- Full Text :
- https://doi.org/10.1063/1.5089796