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[Effects of fibronectin on the monokine production by cultured-human monocytes]
- Source :
- Nihon Rinsho Men'eki Gakkai kaishi = Japanese journal of clinical immunology. 20(2)
- Publication Year :
- 1997
-
Abstract
- The effect of fibronectin (FN) on IL-1 alpha, IL-1 beta, TNF-alpha, and IL-6 production was investigated with cultured monocytes isolated from human peripheral blood. Monokine concentrations were determined by ELISA. FN markedly stimulated the secretion of IL-1 alpha, IL-1 beta, TNF-alpha, and IL-6 from cultured monocytes. Northern blot analysis revealed the up-regulated expression of mRNA specific for each monokine on exposure of monocytes to FN. GM-CSF, IFN-gamma, and LPS synergistically enhanced FN-induced IL-1 alpha production. We further investigated the signal transduction pathways involved in FN-stimulated monokine secretion. FN-stimulated TNF-alpha secretion was markedly inhibited by either herbimycin A or genistein, inhibitors of protein tyrosine kinase (PTK), but was not affected by staurosporin, a inhibitor of protein kinase C (PKC). The results suggest that PTK is required for FN-stimulated TNF-alpha secretion. In contrast, LPS-stimulated TNF-alpha secretion was markedly inhibited by not only herbimycin A or genistein, but also staurosporin. Therefore, both PTK and PKC may be involved in LPS-stimulated TNF-alpha secretion. We also demonstrated that, in monocytes, cytoplasmic proteins of about 70 and 240 kDa were phosphorylated after FN stimulation. Our results indicate that FN may contribute to the inflammatory response of monocyte by inducing monokine production.
- Subjects :
- Lipopolysaccharides
Immunology
Genistein
Monocytes
chemistry.chemical_compound
medicine
Immunology and Allergy
Humans
Secretion
Northern blot
Phosphorylation
Protein kinase C
Cells, Cultured
Protein Kinase C
Chemistry
Monocyte
Monokines
Proteins
Drug Synergism
General Medicine
Protein-Tyrosine Kinases
Molecular biology
Fibronectins
Up-Regulation
Monokine
medicine.anatomical_structure
Cytokines
Signal transduction
Tyrosine kinase
Subjects
Details
- ISSN :
- 09114300
- Volume :
- 20
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Nihon Rinsho Men'eki Gakkai kaishi = Japanese journal of clinical immunology
- Accession number :
- edsair.doi.dedup.....6b574a28e5e43bd1ed766957c770b787