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Distinct hepatic lipid profile of hypertriglyceridemic mice determined by easy ambient sonic-spray ionization mass spectrometry
- Source :
- Analytical and bioanalytical chemistry. 401(5)
- Publication Year :
- 2011
-
Abstract
- Easy ambient sonic-spray ionization mass spectrometry (EASI-MS) was used to interrogate the hepatic lipid profiles of hypertriglyceridemic and control normotriglyceridemic mice. The analyses of ex vivo complex lipid mixtures were made directly with EASI-MS without accompanying separation steps. Intense ions for phosphatidylcholines and triacylglycerols were observed in the positive ion mode whereas the spectra in the negative ion mode provided profiles of phosphatidylethanolamines and phosphatidylinositol. EASI-MS was coupled to high-performance thin-layer chromatography for analysis of free fatty acids. Fourier transform–ion cyclotron resonance–mass spectrometry was also employed to confirm the identity of the detected lipids. We demonstrated higher incorporation of oleic acid in phosphatidylcholine and triacylglycerol composition, higher relative abundance of arachidonic acid containing phosphatidylinositol, and overall distinct free fatty acid profile in the livers of genetic hypertriglyceridemic mice. We propose that these alterations in liver lipid composition are related to the higher tissue and body metabolic rates described in these hypertriglyceridemic mice.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Mice, Transgenic
Mass spectrometry
Biochemistry
Analytical Chemistry
chemistry.chemical_compound
Mice
Phosphatidylcholine
Animals
Humans
Phosphatidylinositol
Triglycerides
Ambient ionization
chemistry.chemical_classification
Hypertriglyceridemia
Chromatography
Fatty acid
Lipid Metabolism
Lipids
Mice, Inbred C57BL
Oleic acid
chemistry
Liver
Arachidonic acid
Ex vivo
Subjects
Details
- ISSN :
- 16182650
- Volume :
- 401
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Analytical and bioanalytical chemistry
- Accession number :
- edsair.doi.dedup.....6b48c275d5bce5663ea0d35ee95c9f64