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Actin-based motility allows Listeria monocytogenes to avoid autophagy in the macrophage cytosol
- Source :
- Cellular microbiology, vol 20, iss 9, Cheng, MI; Chen, C; Engström, P; Portnoy, DA; & Mitchell, G. (2018). Actin-based motility allows Listeria monocytogenes to avoid autophagy in the macrophage cytosol. Cellular Microbiology, 20(9). doi: 10.1111/cmi.12854. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/2wn0d4r0
- Publication Year :
- 2018
- Publisher :
- eScholarship, University of California, 2018.
-
Abstract
- © 2018 John Wiley & Sons Ltd Listeria monocytogenes grows in the host cytosol and uses the surface protein ActA to promote actin polymerisation and mediate actin-based motility. ActA, along with two secreted bacterial phospholipases C, also mediates avoidance from autophagy, a degradative process that targets intracellular microbes. Although it is known that ActA prevents autophagic recognition of L. monocytogenes in epithelial cells by masking the bacterial surface with host factors, the relative roles of actin polymerisation and actin-based motility in autophagy avoidance are unclear in macrophages. Using pharmacological inhibition of actin polymerisation and a collection of actA mutants, we found that actin polymerisation prevented the colocalisation of L. monocytogenes with polyubiquitin, the autophagy receptor p62, and the autophagy protein LC3 during macrophage infection. In addition, the ability of L. monocytogenes to stimulate actin polymerisation promoted autophagy avoidance and growth in macrophages in the absence of phospholipases C. Time-lapse microscopy using green fluorescent protein-LC3 macrophages and a probe for filamentous actin showed that bacteria undergoing actin-based motility moved away from LC3-positive membranes. Collectively, these results suggested that although actin polymerisation protects the bacterial surface from autophagic recognition, actin-based motility allows escape of L. monocytogenes from autophagic membranes in the macrophage cytosol.
- Subjects :
- 0301 basic medicine
DNA Mutational Analysis
medicine.disease_cause
Cytosol
Macrophage
bacteria
Microscopy
Foodborne Illness
Cell biology
Infectious Diseases
Medical Microbiology
Host-Pathogen Interactions
Infection
Intracellular
Immunology
Motility
macromolecular substances
Biology
Filamentous actin
Time-Lapse Imaging
Microbiology
Article
Fluorescence
Vaccine Related
03 medical and health sciences
Motion
Listeria monocytogenes
Bacterial Proteins
xenophagy
Virology
Biodefense
ubiquitin
medicine
Autophagy
Actin
Immune Evasion
PLCs
Macrophages
Prevention
technology, industry, and agriculture
Membrane Proteins
ActA
Actins
030104 developmental biology
Emerging Infectious Diseases
Microscopy, Fluorescence
Mutant Proteins
Protein Multimerization
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Cellular microbiology, vol 20, iss 9, Cheng, MI; Chen, C; Engström, P; Portnoy, DA; & Mitchell, G. (2018). Actin-based motility allows Listeria monocytogenes to avoid autophagy in the macrophage cytosol. Cellular Microbiology, 20(9). doi: 10.1111/cmi.12854. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/2wn0d4r0
- Accession number :
- edsair.doi.dedup.....6b2ec040c8906de821f7df1fce6833f3