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OsDOG1L-3 regulates seed dormancy through the abscisic acid pathway in rice
- Source :
- Plant science : an international journal of experimental plant biology. 298
- Publication Year :
- 2020
-
Abstract
- Seed dormancy is closely related to pre-harvest sprouting resistance. Both plant hormone abscisic acid (ABA) and DELAY OF GERMINATION 1 (DOG1) protein are key regulators of seed dormancy. Their relationship is well reported in Arabidopsis, but little is known in rice. Here, we show that a quantitative trait locus, qSd-1-1 contributes significantly to seed dormancy differences between the strongly dormant indica variety N22 and non-dormant japonica variety Nanjing35. It encodes a DOG1-like protein named OsDOG1L-3 with homology to Arabidopsis DOG1. There were evident promoter and expression differences in OsDOG1L-3 between N22 and Nanjing35, and overexpression or introduction of the N22 OsDOG1L-3 allele in Nanjing35 enhanced its seed dormancy. OsDOG1L-3 expression was positively correlated with seed dormancy and induced by ABA. OsbZIP75 and OsbZIP78 bound directly with the promoter of OsDOG1L-3 to induce its expression. Overexpression of OsbZIP75 increased OsDOG1L-3 protein abundance and promoted seed dormancy. OsDOG1L-3 upregulated expression of ABA-related genes and increased ABA content. We propose that the N22 OsDOG1L-3 allele is a candidate gene for the seed dormancy in QTL qSd-1-1, and that it participates in the ABA pathway to establish seed dormancy in rice.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Quantitative Trait Loci
Gene Expression
Plant Science
Biology
Quantitative trait locus
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Plant Growth Regulators
Arabidopsis
Genetics
Gene
Abscisic acid
Plant Proteins
Oryza sativa
fungi
Seed dormancy
food and beverages
Oryza
General Medicine
biology.organism_classification
Plant Dormancy
Cell biology
030104 developmental biology
chemistry
Germination
Plant hormone
Agronomy and Crop Science
010606 plant biology & botany
Abscisic Acid
Signal Transduction
Subjects
Details
- ISSN :
- 18732259
- Volume :
- 298
- Database :
- OpenAIRE
- Journal :
- Plant science : an international journal of experimental plant biology
- Accession number :
- edsair.doi.dedup.....6b27b42f2dcb7acc81d2652fce77f0b3