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Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
- Source :
- Journal of Applied Microbiology.
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- AIMS Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value-added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost-efficient enzymatic cocktails. METHODS AND RESULTS We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo- and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β-xylosidase, a β-glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. CONCLUSIONS Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. SIGNIFICANCE AND IMPACT OF THE STUDY Cellulomonas sp. B6 could serve as a source of lignocellulose-degrading enzymes applicable to bioprocessing and biotechnological industries.
- Subjects :
- chemistry.chemical_classification
biology
020209 energy
Lignocellulosic biomass
02 engineering and technology
General Medicine
Straw
biology.organism_classification
Lignin
Applied Microbiology and Biotechnology
Cellulomonas sp
Enzyme
Bacterial Proteins
Biochemistry
chemistry
Biofuel
Metabolome
0202 electrical engineering, electronic engineering, information engineering
Glycoside hydrolase
Biomass
Cellulomonas
Bioprocess
Biotechnology
Subjects
Details
- ISSN :
- 13652672 and 13645072
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Microbiology
- Accession number :
- edsair.doi.dedup.....6b0cfd5902ba5fe9a568237a17891ace