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Diversity unearthed by the estimated molecular phylogeny and ecologically quantitative characteristics of uncultured Ehrlichia bacteria in Haemaphysalis ticks, Japan
- Source :
- Scientific Reports, Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
- Publication Year :
- 2020
-
Abstract
- Ehrlichia species are obligatory intracellular bacteria transmitted by arthropods, and some of these species cause febrile diseases in humans and livestock. Genome sequencing has only been performed with cultured Ehrlichia species, and the taxonomic status of such ehrlichiae has been estimated by core genome-based phylogenetic analysis. However, many uncultured ehrlichiae exist in nature throughout the world, including Japan. This study aimed to conduct a molecular-based taxonomic and ecological characterization of uncultured Ehrlichia species or genotypes from ticks in Japan. We first surveyed 616 Haemaphysalis ticks by p28-PCR screening and analyzed five additional housekeeping genes (16S rRNA, groEL, gltA, ftsZ, and rpoB) from 11 p28-PCR-positive ticks. Phylogenetic analyses of the respective genes showed similar trees but with some differences. Furthermore, we found that V1 in the V1–V9 regions of Ehrlichia 16S rRNA exhibited the greatest variability. From an ecological viewpoint, the amounts of ehrlichiae in a single tick were found to equal approx. 6.3E+3 to 2.0E+6. Subsequently, core-partial-RGGFR-based phylogenetic analysis based on the concatenated sequences of the five housekeeping loci revealed six Ehrlichia genotypes, which included potentially new Ehrlichia species. Thus, our approach contributes to the taxonomic profiling and ecological quantitative analysis of uncultured or unidentified Ehrlichia species or genotypes worldwide.
- Subjects :
- 0301 basic medicine
DNA, Bacterial
Science
animal diseases
030106 microbiology
Ehrlichia
Tick
Article
03 medical and health sciences
Ticks
Bacterial Proteins
Japan
parasitic diseases
Animals
Humans
Phylogeny
Genetics
Infectious-disease epidemiology
Multidisciplinary
biology
Phylogenetic tree
Ehrlichiosis
Biodiversity
biology.organism_classification
Haemaphysalis
16S ribosomal RNA
rpoB
bacterial infections and mycoses
Housekeeping gene
030104 developmental biology
Molecular phylogenetics
Medicine
bacteria
Infectious diseases
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....6b015fa6504f10bf6bb54cd70d0252bd