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The genome sequence of the highly acetic acid-tolerant zygosaccharomyces bailii-derived interspecies hybrid strain ISA1307, isolated from a sparkling wine plant
- Source :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP, DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes, DNA Res. 21, 299-313 (2014)
- Publication Year :
- 2014
- Publisher :
- Oxford University Press, 2014.
-
Abstract
- In this work, it is described the sequencing and annotation of the genome of the yeast strain ISA1307, isolated from a sparkling wine continuous production plant. This strain, formerly considered of the Zygosaccharomyces bailii species, has been used to study Z. bailii physiology, in particular, its extreme tolerance to acetic acid stress at low pH. The analysis of the genome sequence described in this work indicates that strain ISA1307 is an interspecies hybrid between Z. bailii and a closely related species. The genome sequence of ISA1307 is distributed through 154 scaffolds and has a size of around 21.2 Mb, corresponding to 96% of the genome size estimated by flow cytometry. Annotation of ISA1307 genome includes 4385 duplicated genes (~90% of the total number of predicted genes) and 1155 predicted single-copy genes. The functional categories including a higher number of genes are 'Metabolism and generation of energy', 'Protein folding, modification and targeting' and 'Biogenesis of cellular components'. The knowledge of the genome sequence of the ISA1307 strain is expected to contribute to accelerate systems-level understanding of stress resistance mechanisms in Z. bailii and to inspire and guide novel biotechnological applications of this yeast species/strain in fermentation processes, given its high resilience to acidic stress. The availability of the ISA1307 genome sequence also paves the way to a better understanding of the genetic mechanisms underlying the generation and selection of more robust hybrid yeast strains in the stressful environment of wine fermentations.<br />This research was supported by FCT and FEDER through POFC-COMPETE [contracts PEst-OE/EQB/ LA0023/2011_ research line: Systems and Synthetic Biology PTDC/AGR-ALI/102608/2008, PEst-C/BIA/ UI4050/2011, and post-doctoral grant to M.P. (SFRH/BPD/73306/2010) and PhD grants to J.F.G. (SFRH/ BD/80065/2011) and F.C.R. (SFRH/BD/82226/2011)]. U.G. acknowledges the Austrian Science Fund (FWF, special research project F3705).
- Subjects :
- Zygosaccharomyces bailii
genome sequencing and annotation
hybrid yeast strains
weak acid food preservatives tolerance
wine yeast strains
Wine
Zygosaccharomyces
Genome
Weak acid food preservatives tolerance
Fungal Proteins
Wine yeast strains
Stress, Physiological
Genetics
Amino Acids
DNA, Fungal
Molecular Biology
Genome size
Gene
Acetic Acid
2. Zero hunger
Whole genome sequencing
Fungal protein
Genome sequencing and annotation
Science & Technology
biology
Base Sequence
Chimera
Chromosome Mapping
Biological Transport
Molecular Sequence Annotation
General Medicine
Full Papers
biology.organism_classification
Genes, Mating Type, Fungal
Yeast
Meiosis
Hybrid yeast strains
Karyotyping
Carbohydrate Metabolism
Genome, Fungal
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP, DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes, DNA Res. 21, 299-313 (2014)
- Accession number :
- edsair.doi.dedup.....6af851eff4d7e594031b90eefd1549d9