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Proton resonance assignments for Pseudomonas aeruginosa ferrocytochrome c-551
Proton resonance assignments for Pseudomonas aeruginosa ferrocytochrome c-551
- Source :
- Biochemical and Biophysical Research Communications. 178:309-314
- Publication Year :
- 1991
- Publisher :
- Elsevier BV, 1991.
-
Abstract
- A comparison between two sets of resonance assignments for ferrocytochrome c-551 from Pseudomonas aeruginosa reveals that major differences can be explained by pH effects. In turn, these reveal side chain protonation events in c-551 that markedly influence spectra. The behavior of resonances in a homologous protein from Pseudomonas stutzeri help to clarify ambiguities in the P. aeruginosa case. A corrected and completed set of proline assignments is presented. Labile side chain protons in residue 47. which hydrogen bonds to the inner heme propionate, appear to be in fast exchange with the solvent.
- Subjects :
- chemistry.chemical_classification
Magnetic Resonance Spectroscopy
Hemeprotein
Proline
biology
Protein Conformation
Stereochemistry
Hydrogen bond
Biophysics
Cytochrome c Group
Protonation
Cell Biology
Hydrogen-Ion Concentration
biology.organism_classification
Biochemistry
Pseudomonas stutzeri
Turn (biochemistry)
Bacterial Proteins
chemistry
Pseudomonas aeruginosa
Side chain
Propionate
Amino Acid Sequence
Molecular Biology
Two-dimensional nuclear magnetic resonance spectroscopy
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 178
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....6aee3555e84b4d8508129341db56bea7
- Full Text :
- https://doi.org/10.1016/0006-291x(91)91815-t