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Proton resonance assignments for Pseudomonas aeruginosa ferrocytochrome c-551

Proton resonance assignments for Pseudomonas aeruginosa ferrocytochrome c-551

Authors :
Mengli Cai
Russell Timkovich
Source :
Biochemical and Biophysical Research Communications. 178:309-314
Publication Year :
1991
Publisher :
Elsevier BV, 1991.

Abstract

A comparison between two sets of resonance assignments for ferrocytochrome c-551 from Pseudomonas aeruginosa reveals that major differences can be explained by pH effects. In turn, these reveal side chain protonation events in c-551 that markedly influence spectra. The behavior of resonances in a homologous protein from Pseudomonas stutzeri help to clarify ambiguities in the P. aeruginosa case. A corrected and completed set of proline assignments is presented. Labile side chain protons in residue 47. which hydrogen bonds to the inner heme propionate, appear to be in fast exchange with the solvent.

Details

ISSN :
0006291X
Volume :
178
Database :
OpenAIRE
Journal :
Biochemical and Biophysical Research Communications
Accession number :
edsair.doi.dedup.....6aee3555e84b4d8508129341db56bea7
Full Text :
https://doi.org/10.1016/0006-291x(91)91815-t