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Influence of Sonication and Taraxacum Officinale Addition on the Antioxidant and Anti-ACE Activity of Protein Extracts from Sous Vide Beef Marinated with Sour Milk and after In Vitro Digestion
- Source :
- Molecules, Volume 25, Issue 20, Molecules, Vol 25, Iss 4692, p 4692 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- The present study assessed the effect of pretreating beef as a raw material for sous vide steak preparation. The pretreatment involved maceration of a batch of meat in sour milk with the simultaneous use of ultrasound (250 or 500 W) as well as the addition of Taraxacum officinale. The biological activity profile of the peptides was assessed in terms of their antioxidant activity and inhibiting activity against angiotensin-converting enzyme (ACE). Changes in the biological activity of peptides under the influence of hydrolysis by gastrointestinal enzymes, i.e., pepsin and pancreatin, were also considered. There was no significant effect of T. officinale addition and sonication of beef batches on the protein content (except for lot S6, after sonication at 500 W as acoustic power and with the addition of dandelion). It was observed that the interaction of maceration in sour milk with simultaneous ultrasound treatment as the initial production step of sous vide beef steak generates the formation of peptides with antioxidant properties. Moreover, peptide formation can be further enhanced by adding dandelion (based on the results of antiradical and chelating activity tests). In addition, the progression of hydrolysis under the influence of gastrointestinal enzymes promotes the release of peptides with antioxidant and anti-ACE activity.
- Subjects :
- sonication
antioxidant
Antioxidant
Taraxacum
030309 nutrition & dietetics
medicine.medical_treatment
Sonication
Pharmaceutical Science
Angiotensin-Converting Enzyme Inhibitors
Dandelion
Article
Antioxidants
Analytical Chemistry
lcsh:QD241-441
dandelion
03 medical and health sciences
Hydrolysis
0404 agricultural biotechnology
lcsh:Organic chemistry
Pepsin
Taraxacum officinale
ACE inhibitor
Animal Proteins, Dietary
Drug Discovery
medicine
Maceration (wine)
Animals
Food science
Physical and Theoretical Chemistry
0303 health sciences
biology
Chemistry
Organic Chemistry
food and beverages
Biological activity
04 agricultural and veterinary sciences
beef
040401 food science
Pepsin A
Red Meat
Milk
Chemistry (miscellaneous)
Fermentation
Pancreatin
biology.protein
Molecular Medicine
bioactive peptides
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....6adb44de1e80e9dbd2b78649f0c024d3
- Full Text :
- https://doi.org/10.3390/molecules25204692