Back to Search
Start Over
'Cytoplasmic domain effects on exposure of co-receptor-binding sites of HIV-1 Env'
- Source :
- Archives of Virology. 161:3011-3018
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- We defined the effects of the cytoplasmic domain (CT) of the Env glycoprotein on co-receptor usage of HIV-1 by reciprocal exchanges of regions containing V3-V5 loops between CD4-dependent and CD4-independent isolates. Primary HIV-1 isolate Env clones CD8 CXCR4-tropic 92UG046 CT84 with an 84-aa truncated CT domain, CD4 CXCR4-tropic 92UG046, and CD4 CCR5-tropic SF162 with full-length (FL) CT domains were used for comparison. The parental 92UG046 Env with CT84 was not fusogenic, but a chimeric SF162 V3-V5-CT84 with an 84-aa truncated CT domain, which demonstrated a switched co-receptor specificity, exhibited syncytium-formation activity with 3T3T4X4 cells. The wild-type (WT) SF162 Env with CT84 or full-length CT was fusogenic in 3T3T4R5 cells. By exchange of V3-V5 loops, we were able to alter WT SF162 to switch its co-receptor preference, which was not dependent on CT domain length. These results provide evidence that CT domains can induce conformational changes in functional regions of gp120 and determine receptor tropism but do not modulate HIV-1 co-receptor specificity.
- Subjects :
- 0301 basic medicine
Protein Conformation
viruses
030106 microbiology
Protein domain
Virus Attachment
Plasma protein binding
HIV Envelope Protein gp120
Biology
Article
Cell Line
Co-receptor binding
03 medical and health sciences
Receptors, HIV
Protein structure
Protein Domains
Virology
Humans
Binding site
Tropism
chemistry.chemical_classification
Binding Sites
virus diseases
General Medicine
Molecular biology
Recombinant Proteins
Viral Tropism
030104 developmental biology
chemistry
HIV-1
Tissue tropism
Mutant Proteins
Glycoprotein
Protein Binding
Subjects
Details
- ISSN :
- 14328798 and 03048608
- Volume :
- 161
- Database :
- OpenAIRE
- Journal :
- Archives of Virology
- Accession number :
- edsair.doi.dedup.....6ac710b45b231fdb8b9e68d833bed1b8