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Protection by Polaprezinc Against Radiation-Induced Apoptosis in Rat Jejunal Crypt Cells
- Source :
- Journal of Radiation Research. 49:341-347
- Publication Year :
- 2008
- Publisher :
- Oxford University Press (OUP), 2008.
-
Abstract
- Polaprezinc, an anti-ulcer drug, is a chelate compound consisting of zinc and L-carnosine. Polaprezinc has been shown to prevent gastric mucosal injury. The anti ulcer effects of polaprezinc have been ascribed to its antioxidative property. The effect of polaprezinc on ionizing radiation-induced apoptosis was studied in the jejunal epithelial crypt cells of rats. Seven-to eight week-old Wistar rats, which were treated with 100 mg/kg of polaprezinc orally 1h before irradiation or 2% carboxymethyl cellulose sodium in controls, were exposed to whole body X-ray irradiation at 2 Gy. The number of apoptotic cells per jejunum crypt was counted in haematoxylin and eosin stained sections at 0-6 h after irradiation. TUNEL positive cells and immunopositive cells for active caspase-3 per crypt were also counted. Accumulation of p53, p21^ and Bax expression in the jejunum after irradiation were examined by Western blot analyses. Polaprezinc treatment given prior to radiation resulted in a significant reduction in numbers of apoptotic cells, TUNEL positive cells and active caspase-3 immunopositive cells in jejunal crypt cells. Polaprezinc treatment resulted in decreases of p53 accumulation, p21^ and Bax expression after irradiation. Polaprezinc has a protective effect against ionizing radiation induced apoptosis in rat jejunal crypt cells.<br />Journal of Radiation Research, 49(4), pp.341-347; 2008
- Subjects :
- Male
p53
Health, Toxicology and Mutagenesis
Crypt
Apoptosis
Radiation-Protective Agents
Polaprezinc
Biology
Haematoxylin
Radiation Dosage
Radiation-induced apoptosis
Jejunum
chemistry.chemical_compound
Western blot
Organometallic Compounds
medicine
Animals
Radiology, Nuclear Medicine and imaging
Rats, Wistar
Cells, Cultured
Radiation
TUNEL assay
Dose-Response Relationship, Drug
Eosin
medicine.diagnostic_test
Carnosine
Dose-Response Relationship, Radiation
Molecular biology
Rats
Intestine
medicine.anatomical_structure
chemistry
Biochemistry
Zinc Compounds
Subjects
Details
- ISSN :
- 13499157 and 04493060
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Journal of Radiation Research
- Accession number :
- edsair.doi.dedup.....6ab64bbc2df2b04bfaa2c0a05e29208b
- Full Text :
- https://doi.org/10.1269/jrr.07114