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Protection by Polaprezinc Against Radiation-Induced Apoptosis in Rat Jejunal Crypt Cells

Authors :
Kumio Okaichi
Masahiro Nakashima
Kazuko Shichijo
Mutsumi Matsuu-Matsuyama
Ichiro Sekine
Takashi Uemura
Daisuke Niino
Toshiyuki Nakayama
Source :
Journal of Radiation Research. 49:341-347
Publication Year :
2008
Publisher :
Oxford University Press (OUP), 2008.

Abstract

Polaprezinc, an anti-ulcer drug, is a chelate compound consisting of zinc and L-carnosine. Polaprezinc has been shown to prevent gastric mucosal injury. The anti ulcer effects of polaprezinc have been ascribed to its antioxidative property. The effect of polaprezinc on ionizing radiation-induced apoptosis was studied in the jejunal epithelial crypt cells of rats. Seven-to eight week-old Wistar rats, which were treated with 100 mg/kg of polaprezinc orally 1h before irradiation or 2% carboxymethyl cellulose sodium in controls, were exposed to whole body X-ray irradiation at 2 Gy. The number of apoptotic cells per jejunum crypt was counted in haematoxylin and eosin stained sections at 0-6 h after irradiation. TUNEL positive cells and immunopositive cells for active caspase-3 per crypt were also counted. Accumulation of p53, p21^ and Bax expression in the jejunum after irradiation were examined by Western blot analyses. Polaprezinc treatment given prior to radiation resulted in a significant reduction in numbers of apoptotic cells, TUNEL positive cells and active caspase-3 immunopositive cells in jejunal crypt cells. Polaprezinc treatment resulted in decreases of p53 accumulation, p21^ and Bax expression after irradiation. Polaprezinc has a protective effect against ionizing radiation induced apoptosis in rat jejunal crypt cells.<br />Journal of Radiation Research, 49(4), pp.341-347; 2008

Details

ISSN :
13499157 and 04493060
Volume :
49
Database :
OpenAIRE
Journal :
Journal of Radiation Research
Accession number :
edsair.doi.dedup.....6ab64bbc2df2b04bfaa2c0a05e29208b
Full Text :
https://doi.org/10.1269/jrr.07114