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Regeneration of partially decellularized tracheal scaffolds in a mouse model of orthotopic tracheal replacement
- Source :
- Journal of Tissue Engineering, Journal of Tissue Engineering, Vol 12 (2021)
- Publication Year :
- 2021
-
Abstract
- Decellularized tracheal scaffolds offer a potential solution for the repair of long-segment tracheal defects. However, complete decellularization of trachea is complicated by tracheal collapse. We created a partially decellularized tracheal scaffold (DTS) and characterized regeneration in a mouse model of tracheal transplantation. All cell populations except chondrocytes were eliminated from DTS. DTS maintained graft integrity as well as its predominant extracellular matrix (ECM) proteins. We then assessed the performance of DTS in vivo. Grafts formed a functional epithelium by study endpoint (28 days). While initial chondrocyte viability was low, this was found to improve in vivo. We then used atomic force microscopy to quantify micromechanical properties of DTS, demonstrating that orthotopic implantation and graft regeneration lead to the restoration of native tracheal rigidity. We conclude that DTS preserves the cartilage ECM, supports neo-epithelialization, endothelialization and chondrocyte viability, and can serve as a potential solution for long-segment tracheal defects.
- Subjects :
- Scaffold
extracellular matrix
Biomedical Engineering
Tracheal collapse
Medicine (miscellaneous)
QD415-436
030204 cardiovascular system & hematology
Biochemistry
Chondrocyte
Partially decellularized tracheal scaffolds
Biomaterials
Extracellular matrix
03 medical and health sciences
0302 clinical medicine
chondrocyte viability
In vivo
medicine
orthotopic tracheal replacement
030304 developmental biology
0303 health sciences
Decellularization
Chemistry
Regeneration (biology)
Cartilage
respiratory system
medicine.anatomical_structure
Original Article
neo-epithelialization
Biomedical engineering
Subjects
Details
- ISSN :
- 20417314
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of tissue engineering
- Accession number :
- edsair.doi.dedup.....6ab329afa498d3e25fb69b07da075092