Back to Search
Start Over
Detection of proviral human T-cell lymphotrophic virus type I DNA in mouthwash samples of HAM/TSP patients and HTLV-I carriers
- Source :
- Scopus-Elsevier
- Publication Year :
- 1996
-
Abstract
- Human T-cell lymphotrophic virus type I (HTLV-I), is a member of the oncogenic retroviruses family endemic in several parts of the world and also recently identified in the Jewish Mashhadi population who immigrated from Iran to Israel. The virus is the causative agent of adult T-cell leukemia (ATL) and a chronic myelopathy known both as tropical spastic paraparesis (TSP) or HTLV-I associated myelopathy (HAM). The known modes of HTLV-I transmission are by sexual intercourse, from mother to child in breast milk, via blood transfusion, and by sharing of needles by parenteral drug users. In the present study we examined the presence of HTLV-I provirus genomic DNA by nested polymerase chain reaction (PCR) and by DNA hybridization in mouthwash samples obtained from 13 Mashhadi-born Iranian Jews with spastic paraparesis associated with HTLV-I, 4 Mashhadi-born Iranian Jews asymptomatic carriers for HTLV-I and 21 healthy controls. Proviral HTLV-I DNA was detected by mouthwash PCR in 12 of 17 HTLV-I infected subjects (71%) but in none of 21 controls. Proviral DNA was also detected in mouthwash samples using HTLV-I probe by dot blot hybridization assay. The presence of HTLV-I proviral DNA in whole saliva may suggest a possible transmission of the virus via saliva and explain the increased rate of infection in elderly Mashhadi-Jewish population.
- Subjects :
- Male
viruses
Population
Molecular Sequence Data
Dot blot
Biology
Iran
Polymerase Chain Reaction
Virus
Proviruses
immune system diseases
Pregnancy
hemic and lymphatic diseases
Virology
Tropical spastic paraparesis
medicine
Ethnicity
Humans
Israel
education
DNA Primers
education.field_of_study
Human T-lymphotropic virus 1
Mouth
Base Sequence
Milk, Human
Infant, Newborn
virus diseases
General Medicine
Provirus
medicine.disease
Infectious Disease Transmission, Vertical
Paraparesis, Tropical Spastic
Jews
Immunology
Carrier State
DNA, Viral
Female
Viral disease
Asymptomatic carrier
Nested polymerase chain reaction
Subjects
Details
- ISSN :
- 03048608
- Volume :
- 141
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Archives of virology
- Accession number :
- edsair.doi.dedup.....6aa983be4ba3679c39e9f58a8c6995f3