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Overexpression of microRNA-129-5p in glioblastoma inhibits cell proliferation, migration, and colony-forming ability by targeting ZFP36L1
- Source :
- Bosnian Journal of Basic Medical Sciences (2019), Bosnian Journal of Basic Medical Sciences
- Publication Year :
- 2019
- Publisher :
- Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina, 2019.
-
Abstract
- Glioblastoma multiforme (GBM) is a highly invasive cancer with a high recurrence rate. The prognosis of GBM patients remains poor, even after standard surgical resection combined with chemoradiotherapy. Thus, there is an urgent need for new therapeutic targets in GBM. In recent years, microRNAs have received considerable attention due to their important role in tumor development and progression. In this study, we investigated the role of miR-129-5p and miR-129-5p/ZFP36L1 axis in GBM tumorigenesis. Analysis of GSE103228 microarray data from the GEO database showed that miR-129-5p was significantly downregulated in GBM vs. normal brain tissues. Quantitative reverse transcription PCR analysis of miR-129-5p expression in seven GBM cell lines (LN229, A172, U87, T98G, U251, H4, and LN118) vs. normal human astrocytes (NHA) showed miR-129-5p was significantly downregulated in GBM cells. Overexpression of miR-129-5p in LN229 and A172 cells significantly suppressed cell proliferation, migration, invasion, and colony-forming ability. Target Scan analysis identified ZFP36L1 as the target of miR-129-5p. UALCAN dataset analysis found that ZFP36L1 was significantly upregulated in GBM vs. normal brain tissues, and high ZFP36L1 expression was positively associated with the poor survival of GBM patients. Western blot analysis demonstrated that ZFP36L1 was significantly upregulated in seven GBM cell lines vs. NHA. Overexpression of miR-129-5p in LN229 and A172 cells significantly inhibited ZFP36L1 mRNA and protein expression, while overexpression of ZFP36L1 in LN229 and A172 cells reversed miR-129-5p-mediated inhibition on GBM tumorigenesis. Our results revealed an important role of miR-129-5p in the negative regulation of ZFP36L1 expression in GBM, suggesting new candidates for targeted therapy in GBM patients.
- Subjects :
- 0301 basic medicine
Cell Survival
medicine.medical_treatment
proliferation
Down-Regulation
medicine.disease_cause
Glioblastoma multiforme
migration
GBM
Targeted therapy
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Cell Movement
Cell Line, Tumor
microRNA
medicine
Humans
Genes, Tumor Suppressor
Neoplasm Invasiveness
U87
Cell Proliferation
Oligonucleotide Array Sequence Analysis
lcsh:R5-920
Microarray analysis techniques
Cell growth
business.industry
Brain Neoplasms
General Medicine
colony-forming ability
tumor-suppressor
nervous system diseases
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
miR-129-5p
Cell culture
030220 oncology & carcinogenesis
Astrocytes
ZFP36L1
Cancer research
Neoplasm Recurrence, Local
Carcinogenesis
business
Butyrate Response Factor 1
Glioblastoma
lcsh:Medicine (General)
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Bosnian Journal of Basic Medical Sciences (2019), Bosnian Journal of Basic Medical Sciences
- Accession number :
- edsair.doi.dedup.....6a7f51b126f9b6275e6f4c882536d793