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Effects of TNF-α and IL-10-819 TC single nucleotide polymorphisms on urogenital schistosomiasis in preschool children in Zimbabwe
- Source :
- African Journal of Laboratory Medicine, African Journal of Laboratory Medicine, Vol 10, Iss 1, Pp e1-e7 (2021)
- Publication Year :
- 2019
-
Abstract
- Background: Knowledge gaps exist between host genetic factors and susceptibility to schistosomiasis. Objective: This study determined cytokine levels and single nucleotide polymorphisms of tumour necrosis factor (TNF)-α (rs1800629) and interleukin (IL)-10 (rs1800871) and their possible impact on susceptibility to schistosomiasis in preschool-age children in the Madziva area of Shamva district, Mashonaland Central province, Zimbabwe. Methods: Urogenital schistosomiasis was diagnosed using the urine filtration method, while a sandwich enzyme-linked immunosorbent assay was used for cytokine level determination. The survey was done in August 2015 and reinfection levels post treatment were assessed at 3, 6 and 12 months. Amplification refractory mutation system polymerase chain reaction with visualisation on 2% agarose gel electrophoresis was used for genotyping. Results: Schistosomiasis prevalence was found to be 10.5% (59/563). Reinfections were detected in only six children at 3 months and only one was reinfected at 12 months. There were no significant differences in TNF-α-308 G/A allele or genotype frequencies between the Schistosoma haematobium infected participants (p = 0.360) and uninfected participants (p = 0.279). However, no children with the IL-10-819 TT genotype had schistosomiasis. The TNF-α GG genotype corresponded with significantly lower TNF-α levels when compared with the GA or AA genotypes (p 0.001), and TNF-α levels were significantly lower in infected children compared to uninfected children (p 0.001). Conclusion: Higher TNF-α levels and lower IL-10 levels are potentially protective against schistosomiasis infection. The IL-10-819 TT genotype is potentially protective against infection through its association with lower IL-10 levels.
- Subjects :
- 030231 tropical medicine
Clinical Biochemistry
Schistosomiasis
Single-nucleotide polymorphism
susceptibility
03 medical and health sciences
protective immunity
0302 clinical medicine
Genotype
medicine
030212 general & internal medicine
Genotyping
Original Research
Schistosoma haematobium
biology
business.industry
Public Health, Environmental and Occupational Health
Interleukin
medicine.disease
biology.organism_classification
cytokines
Genotype frequency
Medical Laboratory Technology
Interleukin 10
Immunology
Public aspects of medicine
RA1-1270
business
polymorphisms
Subjects
Details
- ISSN :
- 22252002
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- African journal of laboratory medicine
- Accession number :
- edsair.doi.dedup.....6a70f901b232f4362b937bae7e47eaf2