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Lymphokine requirement for the generation of natural killer cells from CD34+ hematopoietic progenitor cells
- Source :
- Blood. 85(12)
- Publication Year :
- 1995
-
Abstract
- We have established a cell culture system without stromal cells that allows the CD34+ hematopoietic progenitor cells (HPC) to differentiate into natural killer (NK) cells. CD34+Lin (CD3, CD16, CD56)-cells were purified using fluorescence-activated cell sorting from normal adult bone marrow (BM) and cultured for 28 days in medium supplemented with interleukin-2 (IL-2) and stem cell factor (SCF). NK (CD3-CD16-CD56+) cells were generated in a dose-dependent manner in response to SCF. NK cells originated from CD34+CD33+Lin- cells, but they were barely detectable in cultures of CD34+CD33-Lin- cells. However, on addition of IL-3, an induced differentiation of NK cells from CD34+CD33-Lin- cells was observed, although at a lower frequency. Supplementing of the cell cultures with SCF alone or both SCF and IL-3 for the first 7 days followed by IL-2 for the next 21 days is essential for production of NK cells from CD34+CD33+Lin- cells and from CD34+CD33-Lin- cells, respectively. These data provide direct evidence that NK cells arise from CD34+HPC and show the minimum lymphokine requirement for their differentiation.
- Subjects :
- Immunology
Antigens, CD34
Bone Marrow Cells
Cell Separation
Biology
Hematopoietic Cell Growth Factors
Biochemistry
Natural killer cell
Interleukin 21
Antigens, CD
Bone Marrow
medicine
Humans
Cells, Cultured
Interleukin 3
Stem Cell Factor
Lymphokine-activated killer cell
Cell Differentiation
Cell Biology
Hematology
Natural killer T cell
Flow Cytometry
Hematopoietic Stem Cells
Cell biology
Killer Cells, Natural
medicine.anatomical_structure
Myeloid-derived Suppressor Cell
Interleukin 12
Interleukin-2
Interleukin-3
Stem cell
Subjects
Details
- ISSN :
- 00064971
- Volume :
- 85
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....6a6ec01ba8fe1d49a96b56e64adf2138