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Scavestrogens protect IMR 32 cells from oxidative stress-induced cell death
- Source :
- Toxicology and applied pharmacology. 152(1)
- Publication Year :
- 1998
-
Abstract
- Oxidative stress is considered an important pathophysiological mechanism contributing to promote cell death in a broad variety of diseases including cardiovascular and neurodegenerative disorders. The so-called scavestrogens J811 and J861, structurally derived from 17alpha-estradiol, are potent radical scavengers and inhibitors of iron-induced cell damage in vitro. In this study the potential cytoprotective effects of the scavestrogens J811 and J861 against Fenton reagent-induced cell damage (50 microM FeSO4 plus 200 microM H2O2) were compared with those of 17alpha- and 17beta-estradiol. Cell viability studies using Trypan blue staining showed that estradiols and scavestrogens at concentrations ranging from 0.1 to 10 microM are able to protect IMR 32 neuroblastoma cells from Fenton-mediated death. In addition, these compounds decreased lipid peroxidation measured as thiobarbituric acid reactive substances and renormalize oxidative stress-increased intracellular glutathione levels. When given 6 h after the toxic stimulus, J811 and J861 rescued 60% of cells, whereas 17alpha- and 17beta-estradiol were ineffective. These results suggest that the scavestrogens J811 and J861 are powerful antioxidants capable of interfering with radical-mediated cell death in diseases known to be aggravated by reactive oxygen species. Such compounds may be useful in the development of novel treatments for stroke or neurodegenerative disorders.
- Subjects :
- Programmed cell death
Cell Survival
Iron
Cell
Oxidative phosphorylation
Pharmacology
Toxicology
medicine.disease_cause
Thiobarbituric Acid Reactive Substances
Antioxidants
chemistry.chemical_compound
Neuroblastoma
medicine
Tumor Cells, Cultured
Humans
Viability assay
Cell damage
chemistry.chemical_classification
Reactive oxygen species
Cell Death
Estradiol
Chemistry
Glutathione
Free Radical Scavengers
Hydrogen Peroxide
medicine.disease
Oxidative Stress
medicine.anatomical_structure
Biochemistry
Lipid Peroxidation
Oxidative stress
Subjects
Details
- ISSN :
- 0041008X
- Volume :
- 152
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Toxicology and applied pharmacology
- Accession number :
- edsair.doi.dedup.....6a64e0a1ea5c96e470834071e8a2e2d5