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Type I signal peptidases of Gram-positive bacteria
- Source :
- Biochimica et Biophysica Acta. Molecular Cell Research, 1694, 279-97, Biochimica et Biophysica Acta-Molecular Cell Research, 1694(1-3), 279-297. ELSEVIER SCIENCE BV, Biochimica et Biophysica Acta. Molecular Cell Research, 1694, 1-3, pp. 279-97
- Publication Year :
- 2003
-
Abstract
- Contains fulltext : 59154tjalsma.pdf (Publisher’s version ) (Closed access) Proteins that are exported from the cytoplasm to the periplasm and outer membrane of Gram-negative bacteria, or the cell wall and growth medium of Gram-positive bacteria, are generally synthesized as precursors with a cleavable signal peptide. During or shortly after pre-protein translocation across the cytoplasmic membrane, the signal peptide is removed by signal peptidases. Importantly, pre-protein processing by signal peptidases is essential for bacterial growth and viability. This review is focused on the signal peptidases of Gram-positive bacteria, Bacillus and Streptomyces species in particular. Evolutionary concepts, current knowledge of the catalytic mechanism, substrate specificity requirements and structural aspects are addressed. As major insights in signal peptidase function and structure have been obtained from studies on the signal peptidase LepB of Escherichia coli, similarities and differences between this enzyme and known Gram-positive signal peptidases are highlighted. Notably, while the incentive for previous research on Gram-positive signal peptidases was largely based on their role in the biotechnologically important process of protein secretion, present-day interest in these essential enzymes is primarily derived from the idea that they may serve as targets for novel anti-microbials.
- Subjects :
- POTENTIAL ACTIVE-SITE
Signal peptide
Protein Conformation
STREPTOCOCCUS-PNEUMONIAE
Bacillus
Gram-Positive Bacteria
BACILLUS-SUBTILIS
Bacterial Proteins
CATALYTIC MECHANISM
protein secretion
signal peptide
Molecular Biology
Phylogeny
Molecular diagnosis, prognosis and monitoring [UMCN 1.2]
Signal peptidase
biology
MEMBRANE-PROTEINS
Serine Endopeptidases
Membrane Proteins
Cell Biology
Periplasmic space
BRADYRHIZOBIUM-JAPONICUM
biology.organism_classification
Streptomyces
Transport protein
SECRETORY PRECURSOR
Protein Transport
Secretory protein
Biochemistry
Membrane protein
ESCHERICHIA-COLI
signal peptidase
CYTOPLASMIC MEMBRANE
Bacterial outer membrane
COLI LEADER PEPTIDASE
Bacteria
leader peptidase
Subjects
Details
- ISSN :
- 00063002 and 01674889
- Volume :
- 1694
- Issue :
- 1-3
- Database :
- OpenAIRE
- Journal :
- Biochimica et biophysica acta
- Accession number :
- edsair.doi.dedup.....6a5a58a96944512b593a0fa9e50eb8d8