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Time-lapse deselection model for human day 3 in vitro fertilization embryos: the combination of qualitative and quantitative measures of embryo growth
Time-lapse deselection model for human day 3 in vitro fertilization embryos: the combination of qualitative and quantitative measures of embryo growth
- Source :
- Fertility and sterility. 105(3)
- Publication Year :
- 2015
-
Abstract
- To present a time-lapse deselection model involving both qualitative and quantitative parameters for assessing embryos on day 3.Retrospective cohort study and prospective validation.Private IVF center.A total of 270 embryos with known implantation data (KID) after day 3 transfer from 212 IVF/intracytoplasmic sperm injection (ICSI) cycles were retrospectively analyzed for building the proposed deselection model, followed by prospective validation using an additional 66 KID embryos.None.Morphological score on day 3, embryo morphokinetic parameters, abnormal cleavage patterns, and known implantation results.All included embryos were categorized either retrospectively or prospectively into 7 grades (A+, A, B, C, D, E, F). Qualitative deselection parameters included poor conventional day 3 morphology, abnormal cleavage patterns identified via time-lapse monitoring, and8 cells at 68 hours postinsemination. Quantitative parameters included time from pronuclear fading (PNF) to 5-cell stage and duration of 3-cell stage. KID implantation rates of embryos graded from A+ to F were 52.9%, 36.1%, 25.0%, 13.8%, 15.6%, 3.1%, and 0 respectively (area under the curve [AUC] = 0.762; 95% confidence interval [CI], 0.701-0.824), and a similar pattern was seen in either IVF (AUC = 0.721; 95% CI, 0.622-0.821) or ICSI embryos (AUC = 0.790; 95% CI, 0.711-0.868). Preliminary prospective validation using 66 KID embryos also showed statistically significant prediction in Medicult (AUC = 0.750; 95% CI, 0.588-0.912) and Vitrolife G-Series (AUC = 0.820; 95% CI, 0.671-0.969) suites of culture media.The proposed model involving both qualitative and quantitative deselection effectively predicts day 3 embryo implantation potential and is applicable to all IVF embryos regardless of insemination method by using PNF as the reference starting time point.
- Subjects :
- 0301 basic medicine
Adult
medicine.medical_specialty
Time Factors
medicine.medical_treatment
Fertilization in Vitro
Biology
Insemination
Time-Lapse Imaging
Intracytoplasmic sperm injection
Embryo Culture Techniques
03 medical and health sciences
0302 clinical medicine
Predictive Value of Tests
Pregnancy
medicine
Morphogenesis
Humans
Blastocyst
Embryo Implantation
Prospective Studies
Sperm Injections, Intracytoplasmic
Prospective cohort study
Retrospective Studies
Gynecology
Microscopy
030219 obstetrics & reproductive medicine
In vitro fertilisation
Obstetrics and Gynecology
Reproducibility of Results
Retrospective cohort study
Embryo
Embryo Transfer
Embryo transfer
030104 developmental biology
medicine.anatomical_structure
Fertility
Treatment Outcome
Reproductive Medicine
ROC Curve
Area Under Curve
Infertility
embryonic structures
Female
Subjects
Details
- ISSN :
- 15565653
- Volume :
- 105
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Fertility and sterility
- Accession number :
- edsair.doi.dedup.....6a565ebebe94a6ca9f4f272fff21c1dc