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DJ-1 based peptide, ND-13, promote functional recovery in mouse model of focal ischemic injury

Authors :
Yael Barhum
Tali Ben-Zur
Daniel Offen
Lior Molcho
Source :
PLoS ONE, PLoS ONE, Vol 13, Iss 2, p e0192954 (2018)
Publication Year :
2017

Abstract

Stroke is a leading cause of death worldwide and inflicts serious long-term damage and disability. The vasoconstrictor Endothelin-1, presenting long-term neurological deficits associated with excitotoxicity and oxidative stress is being increasingly used to induce focal ischemic injury as a model of stroke. A DJ-1 based peptide named ND-13 was shown to protect against glutamate toxicity, neurotoxic insults and oxidative stress in various animal models. Here we focus on the benefits of treatment with ND-13 on the functional outcome of focal ischemic injury. Wild type C57BL/6 mice treated with ND-13, after ischemic induction in this model, showed significant improvement in motor function, including improved body balance and motor coordination, and decreased motor asymmetry. We found that DJ-1 knockout mice are more sensitive to Endothelin-1 ischemic insult than wild type mice, contributing thereby additional evidence to the widely reported relevance of DJ-1 in neuroprotection. Furthermore, treatment of DJ-1 knockout mice with ND-13, following Endothelin-1 induced ischemia, resulted in significant improvement in motor functions, suggesting that ND-13 provides compensation for DJ-1 deficits. These preliminary results demonstrate a possible basis for clinical application of the ND-13 peptide to enhance neuroprotection in stroke patients.

Details

ISSN :
19326203
Volume :
13
Issue :
2
Database :
OpenAIRE
Journal :
PloS one
Accession number :
edsair.doi.dedup.....6a5545b5c14f424920250eb0c2f59ea1