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DJ-1 based peptide, ND-13, promote functional recovery in mouse model of focal ischemic injury
- Source :
- PLoS ONE, PLoS ONE, Vol 13, Iss 2, p e0192954 (2018)
- Publication Year :
- 2017
-
Abstract
- Stroke is a leading cause of death worldwide and inflicts serious long-term damage and disability. The vasoconstrictor Endothelin-1, presenting long-term neurological deficits associated with excitotoxicity and oxidative stress is being increasingly used to induce focal ischemic injury as a model of stroke. A DJ-1 based peptide named ND-13 was shown to protect against glutamate toxicity, neurotoxic insults and oxidative stress in various animal models. Here we focus on the benefits of treatment with ND-13 on the functional outcome of focal ischemic injury. Wild type C57BL/6 mice treated with ND-13, after ischemic induction in this model, showed significant improvement in motor function, including improved body balance and motor coordination, and decreased motor asymmetry. We found that DJ-1 knockout mice are more sensitive to Endothelin-1 ischemic insult than wild type mice, contributing thereby additional evidence to the widely reported relevance of DJ-1 in neuroprotection. Furthermore, treatment of DJ-1 knockout mice with ND-13, following Endothelin-1 induced ischemia, resulted in significant improvement in motor functions, suggesting that ND-13 provides compensation for DJ-1 deficits. These preliminary results demonstrate a possible basis for clinical application of the ND-13 peptide to enhance neuroprotection in stroke patients.
- Subjects :
- 0301 basic medicine
Male
Potassium Channels
Physiology
Protein Deglycase DJ-1
Protein Expression
Excitotoxicity
lcsh:Medicine
Cell-Penetrating Peptides
Pharmacology
medicine.disease_cause
Biochemistry
Vascular Medicine
Ion Channels
Brain Ischemia
Brain ischemia
Mice
Oxidative Damage
0302 clinical medicine
Ischemia
Medicine and Health Sciences
Medicine
Vasoconstrictor Agents
lcsh:Science
Stroke
Energy-Producing Organelles
Mice, Knockout
Multidisciplinary
Endothelin-1
Physics
Glutamate receptor
Animal Models
Motor coordination
Mitochondria
Electrophysiology
Neuroprotective Agents
Experimental Organism Systems
Neurology
Knockout mouse
Physical Sciences
Cellular Structures and Organelles
Research Article
Cerebrovascular Diseases
Biophysics
Neurophysiology
Mouse Models
Bioenergetics
Research and Analysis Methods
Neuroprotection
03 medical and health sciences
Model Organisms
Gene Expression and Vector Techniques
Animals
Molecular Biology Techniques
Molecular Biology
Ischemic Stroke
Molecular Biology Assays and Analysis Techniques
business.industry
lcsh:R
Biology and Life Sciences
Proteins
Recovery of Function
Cell Biology
medicine.disease
Peptide Fragments
Mice, Inbred C57BL
Disease Models, Animal
Oxidative Stress
030104 developmental biology
lcsh:Q
business
Reactive Oxygen Species
030217 neurology & neurosurgery
Neuroscience
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....6a5545b5c14f424920250eb0c2f59ea1