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Peptide methionine sulfoxide reductase contributes to the maintenance of adhesins in three major pathogens
- Source :
- Proceedings of the National Academy of Sciences. 93:7985-7990
- Publication Year :
- 1996
- Publisher :
- Proceedings of the National Academy of Sciences, 1996.
-
Abstract
- Pathogenic bacteria rely on adhesins to bind to host tissues. Therefore, the maintenance of the functional properties of these extracellular macromolecules is essential for the pathogenicity of these microorganisms. We report that peptide methionine sulfoxide reductase (MsrA), a repair enzyme, contributes to the maintenance of adhesins in Streptococcus pneumoniae, Neisseria gonorrhoeae, and Escherichia coli. A screen of a library of pneumococcal mutants for loss of adherence uncovered a MsrA mutant with 75% reduced binding to GalNAcbeta1-4Gal containing eukaryotic cell receptors that are present on type II lung cells and vascular endothelial cells. Subsequently, it was shown that an E. coli msrA mutant displayed decreased type I fimbriae-mediated, mannose-dependent, agglutination of erythrocytes. Previous work [Taha, M. K., So, M., Seifert, H. S., Billyard, E. & Marchal, C. (1988) EMBO J. 7, 4367-4378] has shown that mutants with defects in the pilA-pilB locus from N. gonorrhoeae were altered in their production of type IV pili. We show that pneumococcal MsrA and gonococcal PilB expressed in E. coli have MsrA activity. Together these data suggest that MsrA is required for the proper expression or maintenance of functional adhesins on the surfaces of these three major pathogenic bacteria.
- Subjects :
- Guinea Pigs
Molecular Sequence Data
Mutant
Gene Expression
Receptors, Cell Surface
Platelet Membrane Glycoproteins
Biology
Disaccharides
medicine.disease_cause
Polymerase Chain Reaction
Bacterial Adhesion
Pilus
Receptors, G-Protein-Coupled
Microbiology
Escherichia coli
medicine
Animals
Amino Acid Sequence
Adhesins, Bacterial
Peptide sequence
DNA Primers
Multidisciplinary
Base Sequence
Sequence Homology, Amino Acid
Hemagglutination Tests
Neisseria gonorrhoeae
Bacterial adhesin
Streptococcus pneumoniae
Carbohydrate Sequence
Biochemistry
Methionine Sulfoxide Reductases
Methionine sulfoxide reductase
Oxidoreductases
Glycoconjugates
Research Article
MSRA
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 93
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....6a4531ba222abdc5192f2c1c3de2f69f