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Characterization of extensively drug-resistant Mycobacterium tuberculosis in Nepal
- Source :
- Tuberculosis. 93:84-88
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- The emergence of extensively drug-resistant tuberculosis (XDR-TB) has raised public health concern for global control of TB. Although molecular characterization of drug resistance-associated mutations in multidrug-resistant isolates in Nepal has been made, mutations in XDR isolates and their genotypes have not been reported previously. In this study, we identified and characterized 13 XDR Mycobacterium tuberculosis isolates from clinical isolates in Nepal. The most prevalent mutations involved in rifampicin, isoniazid, ofloxacin, and kanamycin/capreomycin resistance were Ser531Leu in rpoB gene (92.3%), Ser315Thr in katG gene (92.3%), Asp94Gly in gyrA gene (53.9%) and A1400G in rrs gene (61.5%), respectively. Spoligotyping and multilocus sequence typing revealed that 69% belonged to Beijing family, especially modern types. Further typing with 26-loci variable number of tandem repeats suggested the current spread of XDR M. tuberculosis. Our result highlights the need to reinforce the TB policy in Nepal with regard to control and detection strategies.
- Subjects :
- Adult
Microbiology (medical)
Tuberculosis
Adolescent
Capreomycin
Extensively Drug-Resistant Tuberculosis
Immunology
Microbial Sensitivity Tests
Minisatellite Repeats
Drug resistance
Biology
Microbiology
Mycobacterium tuberculosis
Young Adult
Nepal
Drug Resistance, Multiple, Bacterial
medicine
Cluster Analysis
Humans
XDR
Phylogeny
Isoniazid
Middle Aged
bacterial infections and mycoses
biology.organism_classification
rpoB
medicine.disease
Virology
Bacterial Typing Techniques
Infectious Diseases
Genes, Bacterial
Mutation
Multilocus sequence typing
Rifampicin
Multilocus Sequence Typing
medicine.drug
Subjects
Details
- ISSN :
- 14729792
- Volume :
- 93
- Database :
- OpenAIRE
- Journal :
- Tuberculosis
- Accession number :
- edsair.doi.dedup.....6a41aa24544e5e0ff5f933883a40c0fc