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Fluorescence lifetime imaging for intraoperative cancer delineation in transoral robotic surgery
- Source :
- Translational biophotonics, Translational biophotonics, vol 1, iss 1-2
- Publication Year :
- 2020
-
Abstract
- This study evaluates the potential for fluorescence lifetime imaging (FLIm) to enhance intraoperative decisionmaking during robotic-assisted surgery of oropharyngeal cancer. Using a custom built FLIm instrument integrated with the da Vinci robotic surgical platform, we first demonstrate that cancer in epithelial tissue diagnosed by histopathology can be differentiated from surrounding healthy epithelial tissue imaged in vivo prior to cancer resection and ex vivo on the excised specimen. Second, we study the fluorescence properties of tissue imaged in vivo at surgical resection margins (tumor bed). Fluorescence lifetimes and spectral intensity ratios were calculated for three spectral channels, producing a set of six FLIm parameters. Current results from 10 patients undergoing TORS procedures demonstrate that healthy epithelium can be resolved from cancer (P < .001) for at least one FLIm parameter. We also showed that a multiparameter linear discriminant analysis approach provides superior discrimination to individual FLIm parameters for tissue imaged both in vivo and ex vivo. Overall, this study highlights the potential for FLIm to be developed into a diagnostic tool for clinical cancer applications of the oropharynx. This technique could help to circumvent the issues posed by the lack of tactile feedback associated with robotic surgical platforms to better enable cancer delineation.<br />Graphical Abstract
- Subjects :
- Fluorescence-lifetime imaging microscopy
surgical visualization
business.industry
Head and neck cancer
Cancer
Bioengineering
autofluorescence
medicine.disease
intraoperative surgical guidance
Article
fluorescence lifetime imaging
Autofluorescence
coregistration techniques
In vivo
Clinical Research
Transoral robotic surgery
robotic surgery
medicine
Biomedical Imaging
Robotic surgery
head and neck cancer
business
Nuclear medicine
Ex vivo
Subjects
Details
- ISSN :
- 26271850
- Volume :
- 1
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Translational biophotonics
- Accession number :
- edsair.doi.dedup.....6a30dd0b8c85bcb3d290f73e48716852