STRUCTURE AND FUNCTION OF PHOTOSYNTHETIC SYSTEMS STUDIED BY HOLE BURNING SPECTROSCOPY

Persistent and transient fluorescence hole-burning spectra of both higher plants and blue-green algae were measured to study the role of structural and functional properties of photosynthetic materials prepared by means of different biochemical methods: intact cells, thylakoide membranes, photosystem II particles and isolated antenna pigments. The width of persistent zero-phonon hole was interpreted due to fast excited energy transfer in antennae. The unstructured transient holes observed on reaction centres of photosystem II particles are connected with a charge-separated bottle-neck.