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A harmonized and standardized in vitro approach produces reliable results on silver nanoparticles toxicity in different cell lines
- Source :
- JAT. Journal of applied toxicology (2021). doi:10.1002/jat.4178, info:cnr-pdr/source/autori:Andreoli C.; Prota V.; De Angelis I.; Facchini E.; Zijno A.; Meccia E.; Barletta B.; Butteroni C.; Corinti S.; Chatgilialoglu C.; Krokidis M.G.; Masi A.; Condello M.; Meschini S.; Di Felice G.; Barone F./titolo:A harmonized and standardized in vitro approach produces reliable results on silver nanoparticles toxicity in different cell lines/doi:10.1002%2Fjat.4178/rivista:JAT. Journal of applied toxicology/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume
- Publication Year :
- 2021
-
Abstract
- Despite the widespread use of silver nanoparticles (AgNPs) in different fields and the amount of investigations available, to date, there are many contradictory results on their potential toxicity. In the present study, extensively characterized 20-nm AgNPs were investigated using optimized protocols and standardized methods to test several toxicological endpoints in different cell lines. The agglomeration/aggregation state of AgNPs in culture media was measured by dynamic light scattering (DLS). DNA and chromosomal damage on BEAS-2B and RAW 264.7 cells were evaluated by comet and micronucleus assays, while oxidative DNA damage by modified comet assay and 8-oxodG/8-oxodA detection. We also investigated immunotoxicity and immunomodulation by cytokine release and NO production in RAW 264.7 and MH-S cells, with or without lipopolysaccharide (LPS) stimulus. Transmission electron microscope (TEM) analysis was used to analyze cellular uptake of AgNPs. Our results indicate different values of AgNPs hydrodynamic diameter depending on the medium, some genotoxic effect just on BEAS-2B and no or slight effects on function of RAW 264.7 and MH-S in absence or presence of LPS stimulus. This study highlights the relevance of using optimized protocols and multiple endpoints to analyze the potential toxicity of AgNPs and to obtain reliable and comparable results.
- Subjects :
- silver nanoparticles
Silver
ultrastructural analysis
Metal Nanoparticles
010501 environmental sciences
In Vitro Techniques
Toxicology
medicine.disease_cause
01 natural sciences
Silver nanoparticle
oxidative DNA damage
Cell Line
03 medical and health sciences
Dynamic light scattering
Toxicity Tests
medicine
Cytotoxicity
030304 developmental biology
0105 earth and related environmental sciences
0303 health sciences
immunotoxicity
Micronucleus Tests
Chemistry
genotoxicity
Comet assay
Cell culture
Toxicity
Biophysics
cytotoxicity
Comet Assay
Micronucleus
characterization in biological media
Genotoxicity
Subjects
Details
- ISSN :
- 10991263
- Volume :
- 41
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of applied toxicology : JATREFERENCES
- Accession number :
- edsair.doi.dedup.....6a26bcfd66ac20df8e90f0415bc5b87b