Back to Search
Start Over
Dynamin Regulates Specific Membrane Fusion Events Necessary for Acrosomal Exocytosis in Mouse Spermatozoa
- Source :
- Journal of Biological Chemistry. 287:37659-37672
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Mammalian spermatozoa must complete an acrosome reaction prior to fertilizing an oocyte. The acrosome reaction is a unique exocytotic event involving a series of prolonged membrane fusions that ultimately result in the production of membrane vesicles and release of the acrosomal contents. This event requires the concerted action of a large number of fusion-competent signaling and scaffolding proteins. Here we show that two different members of the dynamin GTPase family localize to the developing acrosome of maturing mouse germ cells. Both dynamin 1 and 2 also remain within the periacrosomal region of mature mouse spermatozoa and are thus well positioned to regulate the acrosome reaction. Two pharmacological inhibitors of dynamin, dynasore and Dyngo-4a, blocked the in vitro induction of acrosomal exocytosis by progesterone, but not by the calcium ionophore A23187, and elicited a concomitant reduction of in vitro fertilization. In vivo treatment with these inhibitors also resulted in spermatozoa displaying reduced acrosome reaction potential. Dynamin 1 and 2 phosphorylation increased on progesterone treatment, and this was also selectively blocked by dynasore. On the basis of our collective data, we propose that dynamin could regulate specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa.
- Subjects :
- Dynamins
Male
Scaffold protein
endocrine system
Blotting, Western
Acrosome reaction
Fertilization in Vitro
Naphthols
GTPase
Biology
Membrane Fusion
Biochemistry
Dynamin II
Exocytosis
Mice
Testis
Animals
Phosphorylation
Acrosome
Molecular Biology
reproductive and urinary physiology
Dynamin I
Progesterone
Dynamin
Microscopy, Confocal
urogenital system
Acrosome Reaction
Hydrazones
Lipid bilayer fusion
Cell Biology
Spermatozoa
Cell biology
Oocytes
Female
Progestins
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 287
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....6a22286e2af72d179bd6e32612a0377f