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Shark Myelin Basic Protein: Amino Acid Sequence, Secondary Structure, and Self-Association
- Source :
- Journal of Neurochemistry. 55:950-955
- Publication Year :
- 1990
- Publisher :
- Wiley, 1990.
-
Abstract
- Myelin basic protein (MBP) from the Whaler shark (Carcharhinus obscurus) has been purified from acid extracts of a chloroform/methanol pellet from whole brains. The amino acid sequence of the majority of the protein has been determined and compared with the sequences of other MBPs. The shark protein has only 44% homology with the bovine protein, but, in common with other MBPs, it has basic residues distributed throughout the sequence and no extensive segments that are predicted to have an ordered secondary structure in solution. Shark MBP lacks the triproline sequence previously postulated to form a hairpin bend in the molecule. The region containing the putative consensus sequence for encephalitogenicity in the guinea pig contains several substitutions, thus accounting for the lack of activity of the shark protein. Studies of the secondary structure and selfâassociation have shown that shark MBP possesses solution properties similar to those of the bovine protein, despite the extensive differences in primary structure. Copyright
- Subjects :
- Chemical Phenomena
Macromolecular Substances
Protein Conformation
Molecular Sequence Data
chemical and pharmacologic phenomena
Biochemistry
Homology (biology)
Guinea pig
Cellular and Molecular Neuroscience
Myelin
Sequence Homology, Nucleic Acid
Consensus sequence
medicine
Animals
Amino Acid Sequence
Peptide sequence
Protein secondary structure
Brain Chemistry
biology
Chemistry, Physical
Protein primary structure
Lysophosphatidylcholines
Myelin Basic Protein
Myelin basic protein
Molecular Weight
medicine.anatomical_structure
Sharks
biology.protein
Cattle
Ultracentrifugation
human activities
Subjects
Details
- ISSN :
- 14714159 and 00223042
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Journal of Neurochemistry
- Accession number :
- edsair.doi.dedup.....6a0b6eb5912be914d064fd39ead19554