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Involvement of apoptosis in the rat germ cell degeneration induced by nitrobenzene
- Source :
- Archives of Toxicology. 72:296-302
- Publication Year :
- 1998
- Publisher :
- Springer Science and Business Media LLC, 1998.
-
Abstract
- Nitrobenezene (NB) produces germ cell degeneration, especially of spermatocytes in rats. To examine the possible involvement of apoptosis in this process, the extent and nature of nuclear DNA fragmentation after NB dosing were assessed using both terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) and DNA gel electrophoresis, in addition to conventional histological and electron microscopic procedures. Adult Sprague Dawley rats were treated with a single oral dose of NB (250 mg/kg) and euthanized subsequently at 6, 12, and 24 h and 2, 3, 5, and 7 days. The earliest morphological signs of germ cell degeneration in testes were found in pachytene spermatocytes 24 h after dosing. Electron micrographs of degenerating spermatocytes showed marked nuclear chromatin condensation at the nuclear periphery and crowding of cytoplasmic constituents, which are characteristic of apoptosis. Coincident with the appearance of such morphological changes, degenerating spermatocytes contained fragmented DNA as revealed by TUNEL. The presence of DNA laddering, a hallmark of apoptosis on gel electrophoresis, was first apparent and most prominent at 24 h, gradually becoming less detectable. No such changes were observed up to 12 h after dosing or in control animals. These results demonstrated unequivocal involvement of apoptosis in the induction of germ cell degeneration caused by NB.
- Subjects :
- Male
Programmed cell death
Health, Toxicology and Mutagenesis
Apoptosis
DNA Fragmentation
Testicle
Biology
DNA laddering
Toxicology
Rats, Sprague-Dawley
Necrosis
DNA Nucleotidylexotransferase
Spermatocytes
medicine
Animals
Fragmentation (cell biology)
Nitrobenzenes
Electrophoresis, Agar Gel
Gel electrophoresis
TUNEL assay
General Medicine
Molecular biology
Rats
Nuclear DNA
Microscopy, Electron
medicine.anatomical_structure
Genetic Techniques
Immunology
Deoxyuracil Nucleotides
Subjects
Details
- ISSN :
- 14320738 and 03405761
- Volume :
- 72
- Database :
- OpenAIRE
- Journal :
- Archives of Toxicology
- Accession number :
- edsair.doi.dedup.....6a0909acfdda2030f9e137cc0e67b194