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Promoting motor functions in a spinal cord injury model of rats using transplantation of differentiated human olfactory stem cells: A step towards future therapy
- Source :
- Behavioural brain research. 405
- Publication Year :
- 2019
-
Abstract
- Human olfactory ecto-mesenchymal stem cells (hOE-MSCs) derived from the human olfactory mucosa (OM) can be easily isolated and expanded in cultures while their immense plasticity is maintained. To mitigate ethical concerns, the hOE-MSCs can be also transplanted across allogeneic barriers, making them desirable cells for clinical applications. The main purpose of this study was to evaluate the effects of administering the hOE-MSCs on a spinal cord injury (SCI) model of rats. These cells were accordingly isolated and cultured, and then treated in the neurobasal medium containing serum-free Dulbecco’s Modified Essential Medium (DMEM) and Ham’s F-12 Medium (DMEM/F12) with 2% B27 for two days. Afterwards, the pre-induced cells were incubated in N2B27 with basic fibroblast growth factor (bFGF), fibroblast growth factor 8b (FGF8b), sonic hedgehog (SHH), and ascorbic acid (vitamin C) for six days. The efficacy of the induced cells was additionally evaluated using immunocytochemistry (ICC) and real-time polymerase chain reaction (RT-PCR). The differentiated cells were similarly transplanted into the SC contusions. Functional recovery was further conducted on a weekly basis for eight consecutive weeks. Moreover, cell integration was assessed via conventional histology and ICC, whose results revealed the expression of choline acetyltransferase (ChAT) marker at the induction stage. According to the RT-PCR findings, the highest expression level of insulin gene-enhancer protein (islet-1), oligodendrocyte transcription factor (Olig2), and homeobox protein HB9 was observed at the induction stage. The number of engraftment cells also rose (approximately by 2.5 % ± 0.1) in the motor neuron-like cells derived from the hOE-MSCs-grafted group compared with the OE-MSCs-grafted one. The functional analysis correspondingly revealed that locomotor and sensory scores considerably improved in the rats in the treatment group. These findings suggested that motor neuron-like cells derived from the hOE-MSCs could be utilized as an alternative cell-based therapeutic strategy for SCI.
- Subjects :
- Male
Cellular differentiation
Basic fibroblast growth factor
Biology
Mesenchymal Stem Cell Transplantation
Andrology
OLIG2
Rats, Sprague-Dawley
03 medical and health sciences
Behavioral Neuroscience
chemistry.chemical_compound
Olfactory mucosa
0302 clinical medicine
Olfactory Mucosa
medicine
Animals
Humans
Cells, Cultured
Spinal Cord Injuries
030304 developmental biology
Motor Neurons
0303 health sciences
Behavior, Animal
Ascorbic acid
Oligodendrocyte
Rats
Transplantation
Disease Models, Animal
medicine.anatomical_structure
chemistry
Stem cell
030217 neurology & neurosurgery
Locomotion
Subjects
Details
- ISSN :
- 18727549
- Volume :
- 405
- Database :
- OpenAIRE
- Journal :
- Behavioural brain research
- Accession number :
- edsair.doi.dedup.....69e84800ac047f48f45b96df87289379