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Efficient Transendothelial Migration of Latently HIV-1-Infected Cells
- Source :
- Viruses, Volume 13, Issue 8, Viruses, Vol 13, Iss 1589, p 1589 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- A small fraction of HIV-1-infected T cells forms populations of latently infected cells when they are a naive T-cell subset or in transit to a resting memory state. Latently HIV-1-infected cells reside in lymphoid tissues and serve as viral reservoirs. However, whether they systemically recirculate in the body and re-enter the lymphoid nodes are unknown. Here, we employed two in-vitro cell coculture systems mimicking the lymphatic endothelium in lymph nodes and investigated the homing potential, specifically the transendothelial migration (TEM), of two latently HIV-1-infected cell lines (J1.1 and ACH-2). In trans-well coculture systems, J1.1 and ACH-2 showed higher TEM efficiencies than their parental uninfected and acutely infected cells. The efficiency of TEM was enhanced by the presence of stromal cells, such as HS-5 and fibroblastic reticular cells. In an in-vitro reconstituted, three-dimensional coculture system in which stromal cells are embedded in collagen matrices, J1.1 showed slightly higher TEM efficiency in the presence of HS-5. In accordance with these phenotypes, latently infected cells adhered to the endothelial cells more efficiently than uninfected cells. Together, our study showed that latently HIV-1-infected cells enhanced cell adhesion and TEM abilities, suggesting their potential for efficient homing to lymph nodes.
- Subjects :
- CD4-Positive T-Lymphocytes
latent infection
Stromal cell
government.form_of_government
Receptors, Lymphocyte Homing
Microbiology
Article
Cell Line
Reticular cell
Virology
Humans
Cell adhesion
Lymphocyte homing receptor
transmigration
stromal cells
Chemistry
Transendothelial and Transepithelial Migration
Endothelial Cells
QR1-502
Coculture Techniques
Cell biology
Virus Latency
Lymphatic Endothelium
Infectious Diseases
Cell culture
government
reconstitution
HIV-1
Virus Activation
Lymph
lymphocyte homing
Homing (hematopoietic)
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....69cb51cdfd60dd149afc231a11a0d7dd
- Full Text :
- https://doi.org/10.3390/v13081589