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Red Blood Cell Membrane as a Biomimetic Nanocoating for Prolonged Circulation Time and Reduced Accelerated Blood Clearance
- Source :
- Small (Weinheim an der Bergstrasse, Germany). 11(46)
- Publication Year :
- 2015
-
Abstract
- For decades, poly(ethylene glycol) (PEG) has been widely incorporated into nanoparticles for evading immune clearance and improving the systematic circulation time. However, recent studies have reported a phenomenon known as "accelerated blood clearance (ABC)" where a second dose of PEGylated nanomaterials is rapidly cleared when given several days after the first dose. Herein, we demonstrate that natural red blood cell (RBC) membrane is a superior alternative to PEG. Biomimetic RBC membrane-coated Fe(3)O(4) nanoparticles (Fe(3)O(4) @RBC NPs) rely on CD47, which is a "don't eat me" marker on the RBC surface, to escape immune clearance through interactions with the signal regulatory protein-alpha (SIRP-α) receptor. Fe(3)O(4) @RBC NPs exhibit extended circulation time and show little change between the first and second doses, with no ABC suffered. In addition, the administration of Fe(3)O(4) @RBC NPs does not elicit immune responses on neither the cellular level (myeloid-derived suppressor cells (MDSCs)) nor the humoral level (immunoglobulin M and G (IgM and IgG)). Finally, the in vivo toxicity of these cell membrane-camouflaged nanoparticles is systematically investigated by blood biochemistry, hematology testing, and histology analysis. These findings are significant advancements toward solving the long-existing clinical challenges of developing biomaterials that are able to resist both immune response and rapid clearance.
- Subjects :
- Materials science
Time Factors
Cell
Static Electricity
Pharmacology
Ferric Compounds
Polyethylene Glycols
Biomaterials
chemistry.chemical_compound
Mice
Immune system
Coated Materials, Biocompatible
Biomimetic Materials
PEG ratio
Materials Testing
medicine
Animals
General Materials Science
Tissue Distribution
Receptor
Immune Evasion
biology
CD47
Macrophages
Erythrocyte Membrane
General Chemistry
Red blood cell
medicine.anatomical_structure
RAW 264.7 Cells
chemistry
Immunoglobulin M
Immunology
Blood Circulation
biology.protein
Hydrodynamics
Nanoparticles
Ethylene glycol
Biotechnology
Subjects
Details
- ISSN :
- 16136829
- Volume :
- 11
- Issue :
- 46
- Database :
- OpenAIRE
- Journal :
- Small (Weinheim an der Bergstrasse, Germany)
- Accession number :
- edsair.doi.dedup.....69c6bba05fd3df7f9daa32f2bc819a03