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Fast retrograde access to projection neuron circuits underlying vocal learning in songbirds
- Publication Year :
- 2020
- Publisher :
- Cold Spring Harbor Laboratory, 2020.
-
Abstract
- SummaryUnderstanding the structure and function of neural circuits underlying speech and language is a vital step towards better treatments for diseases of these systems. Songbirds, among the few animal orders that share with humans the ability to learn vocalizations from a conspecific, have provided many insights into the neural mechanisms of vocal development. However, research into vocal learning circuits has been hindered by a lack of tools for rapid genetic targeting of specific neuron populations to meet the quick pace of developmental learning. Here, we present a new viral tool that enables fast and efficient retrograde access to projection neuron populations. In zebra finches, Bengalese finches, canaries, and mice, we demonstrate fast retrograde labeling of cortical or dopaminergic neurons. We further demonstrate the suitability of our construct for detailed morphological analysis, for in vivo imaging of calcium activity, and for multicolor brainbow labeling.
- Subjects :
- 0303 health sciences
Computer science
Dopaminergic
Projection neuron
03 medical and health sciences
0302 clinical medicine
medicine.anatomical_structure
nervous system
medicine
Biological neural network
Brainbow
Vocal learning
Neuron
Neuroscience
030217 neurology & neurosurgery
030304 developmental biology
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....69b2a56b1f29e14c7e5d42b58fdbab0f
- Full Text :
- https://doi.org/10.1101/2020.02.19.955773