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The effect of 1,25-dihydroxyvitamin D3 treatment on calcitonin and calcitonin gene-related peptide mRNA levels in cultured human thyroid C-cells
- Source :
- Biochemical and Biophysical Research Communications. 149:239-243
- Publication Year :
- 1987
- Publisher :
- Elsevier BV, 1987.
-
Abstract
- We utilized the TT cell, a human C-cell line derived from a medullary thyroid carcinoma, to study the effects of 1,25-dihydroxyvitamin D3 on cell growth and expression of the calcitonin gene. The growth rate of cells treated for 8 days with 1,25-dihydroxyvitamin D3 did not differ significantly from control or 24,25-dihydroxyvitamin D3 treated cells. Total RNA was isolated, and calcitonin and calcitonin gene-related peptide (CGRP) levels were measured by hybridization. 1,25 D3 lowered calcitonin and CGRP mRNA levels in a time- and dose-dependent fashion; 24,25 D3 had no effect. Northern blots revealed a decrease in the mature mRNA as well as the common precursor forms, indicating a transcriptional effect of 1,25 D3.
- Subjects :
- Calcitonin
medicine.medical_specialty
24,25-Dihydroxyvitamin D 3
Calcitriol
Calcitonin Gene-Related Peptide
Cell
Biophysics
Calcitonin gene-related peptide
Biochemistry
Thyroid carcinoma
Internal medicine
Tumor Cells, Cultured
medicine
Humans
RNA, Messenger
Thyroid Neoplasms
Northern blot
Calcitonin receptor
Molecular Biology
Chemistry
Cell growth
Carcinoma
Neuropeptides
Nucleic Acid Hybridization
Cell Biology
Molecular biology
Kinetics
Endocrinology
medicine.anatomical_structure
Dihydroxycholecalciferols
medicine.drug
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 149
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....69a8c18ecce2e3daa692d79eec91d0c2