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Non-Invasive versus Invasive Samples for Zika Virus Surveillance: A Comparative Study in New Caledonia and French Guiana in 2015-2016
- Source :
- Microorganisms, Microorganisms, MDPI, 2021, 9 (6), pp.1312. ⟨10.3390/microorganisms9061312⟩, Volume 9, Issue 6, Microorganisms, Vol 9, Iss 1312, p 1312 (2021)
- Publication Year :
- 2021
-
Abstract
- International audience; Zika virus, an arbovirus responsible for major outbreaks, can cause serious health issues, such as neurological diseases. In the present study, different types of samples (serum, saliva, and urine), collected in 2015–2016 in New Caledonia and French Guiana from 53 patients presenting symptoms and clinical signs triggered by arbovirus infections, were analyzed using a recently developed, and in-house validated, 4-plex RT-qPCR TaqMan method for simultaneous detection and discrimination of the Zika and Chikungunya viruses. Subsequently, statistical analyses were performed in order to potentially establish recommendations regarding the choice of samples type to use for an efficient and early stage Zika infection diagnosis. On this basis, the use of only urine samples presented the highest probability to detect viral RNA from Zika virus. Moreover, such a probability was improved using both urine and saliva samples. Consequently, the added value of non-invasive samples, associated with a higher acceptance level for collection among patients, instead of serum samples, for the detection of Zika infections was illustrated.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
QH301-705.5
Arbovirus Infections
public health concern
030106 microbiology
diagnostic
medicine.disease_cause
Microbiology
Arbovirus
Article
Zika virus
03 medical and health sciences
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Virology
medicine
TaqMan
Chikungunya
Biology (General)
biology
business.industry
RT-qPCR
Non invasive
Outbreak
Serum samples
biology.organism_classification
medicine.disease
3. Good health
030104 developmental biology
business
serum
urine and saliva samples
Subjects
Details
- ISSN :
- 20762607
- Volume :
- 9
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....6972e37cddc7f7ee41c4f785aa0a00e4