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A yeast exosome cofactor, Mpp6, functions in RNA surveillance and in the degradation of noncoding RNA transcripts
- Source :
- Molecular and Cellular Biology, Molecular and Cellular Biology, American Society for Microbiology, 2008, 28 (17), pp.5446--5457. ⟨10.1128/MCB.00463-08⟩, Molecular and Cellular Biology, 2008, 28 (17), pp.5446--5457. ⟨10.1128/MCB.00463-08⟩, Milligan, L, Decourty, L, Saveanu, C, Rappsilber, J, Ceulemans, H, Jacquier, A & Tollervey, D 2008, ' A yeast exosome cofactor, Mpp6, functions in RNA surveillance and in the degradation of noncoding RNA transcripts ', Molecular and Cellular Biology, vol. 28, no. 17, pp. 5446-5457 . https://doi.org/10.1128/MCB.00463-08
- Publication Year :
- 2008
-
Abstract
- A genome-wide screen for synthetic lethal (SL) interactions with loss of the nuclear exosome cofactors Rrp47/Lrp1 or Air1 identified 335 exonucleases, the THO complex required for mRNP assembly, and Ynr024w (Mpp6). SL interactions with mpp6 were confirmed for rrp47 and nuclear exosome component Rrp6. The results of bioinformatic analyses revealed homology between Mpp6 and a human exosome cofactor, underlining the high conservation of the RNA surveillance system. Mpp6 is an RNA binding protein that physically associates with the exosome and was localized throughout the nucleus. The results of functional analyses demonstrated roles for Mpp6 in the surveillance of both pre-rRNA and pre-mRNAs and in the degradation of “cryptic” noncoding RNAs (ncRNAs) derived from intergenic regions and the ribosomal DNA spacer heterochromatin. Strikingly, these ncRNAs are also targeted by other exosome cofactors, including Rrp47, the TRAMP complex (which includes Air1), and the Nrd1/Nab3 complex, and are degraded by both Rrp6 and the core exosome. Heterochromatic transcripts and other ncRNAs are characterized by very rapid degradation, and we predict that functional redundancy is an important feature of ncRNA metabolism. The results of genetic and biochemical analyses indicate that the exosome 3-to-5 exonuclease complex has potent in vivo RNA degradation activity, even on highly structured RNAs and large RNA-protein (RNP) complexes. However, the purified eukaryotic exosome shows limited activity in vitro, and this
- Subjects :
- Exonuclease
RNA Stability
RNA Processing
RNA, Untranslated
Saccharomyces cerevisiae Proteins
Exosome complex
[SDV]Life Sciences [q-bio]
Messenger
Molecular Sequence Data
Sequence Homology
Post-Transcriptional
RNA-binding protein
Saccharomyces cerevisiae
Exosome
03 medical and health sciences
0302 clinical medicine
Gene Expression Regulation, Fungal
RNA Precursors
Humans
Amino Acid Sequence
RNA, Messenger
RNA Processing, Post-Transcriptional
Molecular Biology
030304 developmental biology
Genetics
Cell Nucleus
0303 health sciences
biology
Sequence Homology, Amino Acid
RNA
Untranslated
RNA-Binding Proteins
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
RNA, Fungal
Cell Biology
Articles
Non-coding RNA
Cell biology
Amino Acid
Fungal
Gene Expression Regulation
TRAMP complex
Mutation
biology.protein
030217 neurology & neurosurgery
Protein Binding
Subjects
Details
- ISSN :
- 10985549 and 02707306
- Volume :
- 28
- Issue :
- 17
- Database :
- OpenAIRE
- Journal :
- Molecular and cellular biology
- Accession number :
- edsair.doi.dedup.....6947abba1997610cbc6f01eb3bb76487