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The clinical value of measuring circulating hpv dna during chemo-radiotherapy in squamous cell carcinoma of the anus
- Source :
- Lefèvre, A C, Pallisgaard, N, Kronborg, C, Wind, K L, Krag, S R P & Spindler, K L G 2021, ' The clinical value of measuring circulating hpv dna during chemo-radiotherapy in squamous cell carcinoma of the anus ', Cancers, vol. 13, no. 10, 2451 . https://doi.org/10.3390/cancers13102451, Cancers, Vol 13, Iss 2451, p 2451 (2021), Cancers, Volume 13, Issue 10
- Publication Year :
- 2021
-
Abstract
- Background and purpose: Circulating tumor DNA (ctDNA) is investigated in various cancers. In squamous cell carcinoma of the anus (SCCA) infection with human papilloma virus (HPV) is found in around 90% of cases and here, plasma HPV (pHPV) can be used as ctDNA. Preliminary data have proved the ability to detect pHPV16 and -18 in SCCA. We have developed a highly sensitive method for measurement of six relevant pHPV subtypes, to investigate the elimination pattern of pHPV during chemo-radiotherapy (CRT) for SCCA and its clinical value. Material and methods: Patients treated at Aarhus University Hospital from 2016–2020 were included. P16 status in the primary biopsy was measured and 82% of patients had P16 positive tumor. Blood samples were collected prior to treatment (PT), mid treatment (MT), end of therapy (EOT), and during follow-up (FU). An in-house multiplex digital droplet PCR method measured pHPV subtypes 16, 18, 31, 33, 51, 58. Results: Samples from 88 patients were drawn PT (n = 73), MT (n = 72), EOT (n = 64) and during FU (n = 41). Plasma HPV was detectable in 52 patients and PT pHPV levels correlated to tumor stages. Three elimination patterns were observed during CRT with correlation to outcome: fast responders with no local or distant failures (0/12)<br />slow responders with high risk of local failures (4/20), no distant failures<br />persistent molecular responders with high risk of distant failures (4/13), but no local failures, p &lt<br />0.01. Conclusion: During CRT, pHPV can divide patients with SCCA into three groups with significantly different risk of failure. The use of pHPV can potentially assist in clinical treatment decision.
- Subjects :
- squamous cell carcinoma
0301 basic medicine
Oncology
Cancer Research
medicine.medical_specialty
Human papillomavirus
Article
03 medical and health sciences
0302 clinical medicine
Internal medicine
Squamous cell carcinoma
Biopsy
medicine
Basal cell
Anus neoplasms
human papillomavirus
RC254-282
circulating tumor DNA
Chemo-radiotherapy
Circulating tumor DNA
medicine.diagnostic_test
business.industry
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Anus
Hpv testing
030104 developmental biology
medicine.anatomical_structure
anus neoplasms
ELIMINATION PATTERNS
030220 oncology & carcinogenesis
Clinical value
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Lefèvre, A C, Pallisgaard, N, Kronborg, C, Wind, K L, Krag, S R P & Spindler, K L G 2021, ' The clinical value of measuring circulating hpv dna during chemo-radiotherapy in squamous cell carcinoma of the anus ', Cancers, vol. 13, no. 10, 2451 . https://doi.org/10.3390/cancers13102451, Cancers, Vol 13, Iss 2451, p 2451 (2021), Cancers, Volume 13, Issue 10
- Accession number :
- edsair.doi.dedup.....69418b45711c564bad7c5cb55bfaa3fd
- Full Text :
- https://doi.org/10.3390/cancers13102451