Back to Search
Start Over
Effects of substance P on osteoblastic differentiation and heme oxygenase-1 in human periodontal ligament cells
- Source :
- Cell biology international. 33(3)
- Publication Year :
- 2008
-
Abstract
- Although substance P (SP) is associated with osteoclast differentiation and bone resorption, little is known about the osteogenic differentiation-inducing effects of SP in periodontal ligament (PDL) cells. This study investigated whether PDL cells could differentiate into osteoblastic-like cells by SP. The expression of osteoblastic differentiation markers such as osteopontin (OPN), osteonectin (ON), osteocalcin (OCN) and bone sialoprotein (BSP) were evaulated by Western blotting. Additionally, SP-mediated heme oxygenase-1 (HO-1) pathways were further clarified. SP increased HO-1 and osteogenic differentiation in concentration- and time-dependent manners, as determined by OPN, ON, OCN and BSP expression. Furthermore, treatment with inhibitors of p38, ERK MAPK, and NF-κB abolished SP-induced osteogenic differentiation and HO-1 expression. SP-induced translocation of Nrf-2 was also observed. The combined results suggest that SP activates the stress-response enzymes HO-1 and Nrf-2, subsequently leading to upregulation of osteogenic differentiation in human PDL cells.
- Subjects :
- Bone sialoprotein
NF-E2-Related Factor 2
Periodontal Ligament
Sialoglycoproteins
Osteocalcin
Substance P
Bone resorption
stomatognathic system
Osteoclast
medicine
Periodontal fiber
Humans
Integrin-Binding Sialoprotein
Osteonectin
Osteopontin
Enzyme Inhibitors
Cell Line, Transformed
Osteoblasts
biology
Chemistry
Cell Differentiation
Cell Biology
General Medicine
Molecular biology
Up-Regulation
Heme oxygenase
medicine.anatomical_structure
Immunology
biology.protein
Heme Oxygenase-1
Subjects
Details
- ISSN :
- 10958355
- Volume :
- 33
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Cell biology international
- Accession number :
- edsair.doi.dedup.....691c1052d66dc2b393a3723595f31aea