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Investigation of the antibiofilm capacity of peptide-modified stainless steel
- Source :
- Royal Society Open Science, Royal Society Open Science, Vol 5, Iss 3 (2018)
- Publication Year :
- 2017
-
Abstract
- Biofilm formation on surfaces is an important research topic in ship tribology and medical implants. In this study, dopamine and two types of synthetic peptides were designed and attached to 304 stainless steel surfaces, aiming to inhibit the formation of biofilms. A combinatory surface modification procedure was applied in which dopamine was used as a coupling agent, allowing a strong binding ability with the two peptides. X-ray photoelectron spectroscopy (XPS), elemental analysis, contact angle measurement and surface roughness test were used to evaluate the efficiency of the peptide modification. An antibiofilm assay against Staphylococcus aureus was conducted to validate the antibiofilm capacity of the peptide-modified stainless steel samples. XPS analysis confirmed that the optimal dopamine concentration was 40 µg ml −1 in the coupling reaction. Element analysis showed that dopamine and the peptides had bound to the steel surfaces. The robustness assay of the modified surface demonstrated that most peptide molecules had bound on the surface of the stainless steel firmly. The contact angle of the modified surfaces was significantly changed. Modified steel samples exhibited improved antibiofilm properties in comparison to untreated and dopamine-only counterpart, with the peptide 1 modification displaying the best antibiofilm effect. The modified surfaces showed antibacterial capacity. The antibiofilm capacity of the modified surfaces was also surface topography sensitive. The steel sample surfaces polished with 600# sandpaper exhibited stronger antibiofilm capacity than those polished with other types of sandpapers after peptide modification. These findings present valuable information for future antifouling material research.
- Subjects :
- Materials science
Peptide
02 engineering and technology
Q1
010402 general chemistry
01 natural sciences
Biochemistry and Biophysics
Biofouling
Contact angle
X-ray photoelectron spectroscopy
topography
synthetic peptide
Surface roughness
lcsh:Science
stainless steel
Sandpaper
chemistry.chemical_classification
Peptide modification
Multidisciplinary
antibiofilm
technology, industry, and agriculture
021001 nanoscience & nanotechnology
R1
0104 chemical sciences
Chemical engineering
chemistry
Surface modification
lcsh:Q
dopamine
0210 nano-technology
surface modification
Research Article
Subjects
Details
- ISSN :
- 20545703
- Volume :
- 5
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Royal Society open science
- Accession number :
- edsair.doi.dedup.....691648a46fedf9688f0a2981c8cabc65