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A 25-Residue Peptide From Botrytis cinerea Xylanase BcXyn11A Elicits Plant Defenses
- Source :
- Frontiers in Plant Science, Vol 10 (2019)
- Publication Year :
- 2019
- Publisher :
- Frontiers Media S.A., 2019.
-
Abstract
- Plants activate defense responses against a possible pathogen once pattern-recognition receptors (PRRs) perceive the presence of pathogen-associated molecular patterns (PAMPs). Glycosyl hydrolase family 11 (GH11) endoxylanases from Trichoderma, Fusarium and Botrytis species have been described as being able to induce the defense response in plants, in a way that is independent of its enzymatic activity. However, until now, it has not been possible to establish with certainty which regions of these enzymes are recognized by plants as PAMPs. We show here for the first time that a short 25-residue peptide (named Xyn25) from the Botrytis cinerea xylanase BcXyn11A can reproduce by itself all the effects observed for the treatment of plants with the whole BcXyn11A protein. These include necrosis on leaves, seedling growth inhibition, induction of a ROS burst, electrolyte leakage, cytoplasm shrinkage, autofluorescence, cell death, and induction of defense genes. Two highly conserved four-amino acid regions within Xyn25 were shown to be necessary for the elicitation activity by substituting them with tracts of four alanine residues.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Plant Science
lcsh:Plant culture
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
plant defense
Plant defense against herbivory
Glycosyl
lcsh:SB1-1110
Gene
Botrytis cinerea
Alanine
xylanase
elicitor
biology
food and beverages
PAMP
biology.organism_classification
Elicitor
030104 developmental biology
hypersensitive response (HR)
Biochemistry
chemistry
Trichoderma
Xylanase
010606 plant biology & botany
Subjects
Details
- Language :
- English
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Frontiers in Plant Science
- Accession number :
- edsair.doi.dedup.....6901fe437673df985a21c3229983e4de