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Determining Lineage Pathways from Cellular Barcoding Experiments
- Source :
- Cell Reports, Vol 6, Iss 4, Pp 617-624 (2014), Cell Reports [E], 6(4), 617. Elsevier Saunders, Cell Reports, Cell Reports, Elsevier Inc, 2014, 6 (4), pp.617-624. ⟨10.1016/j.celrep.2014.01.016⟩
- Publication Year :
- 2014
- Publisher :
- Elsevier, 2014.
-
Abstract
- SummaryCellular barcoding and other single-cell lineage-tracing strategies form experimental methodologies for analysis of in vivo cell fate that have been instrumental in several significant recent discoveries. Due to the highly nonlinear nature of proliferation and differentiation, interrogation of the resulting data for evaluation of potential lineage pathways requires a new quantitative framework complete with appropriate statistical tests. Here, we develop such a framework, illustrating its utility by analyzing data from barcoded multipotent cells of the blood system. This application demonstrates that the data require additional paths beyond those found in the classical model, which leads us to propose that hematopoietic differentiation follows a loss of potential mechanism and to suggest further experiments to test this deduction. Our quantitative framework can evaluate the compatibility of lineage trees with barcoded data from any proliferating and differentiating cell system.
- Subjects :
- [SDV]Life Sciences [q-bio]
Computational biology
Cell lineage
Biology
Cell fate determination
Bioinformatics
Biological
Hematopoietic Stem Cells
Models, Biological
General Biochemistry, Genetics and Molecular Biology
Cell system
Hematopoiesis
Mice
lcsh:Biology (General)
Models
Animals
Cell Lineage
Potential mechanism
lcsh:QH301-705.5
ComputingMilieux_MISCELLANEOUS
Hamilton Institute
Subjects
Details
- Language :
- English
- ISSN :
- 22111247
- Volume :
- 6
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cell Reports
- Accession number :
- edsair.doi.dedup.....68ff070904e2ba85e42544106aae23b1