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Performance of a SARS CoV-2 antibody ELISA based on simultaneous measurement of antibodies against the viral nucleoprotein and receptor-binding domain
- Source :
- European Journal of Clinical Microbiology & Infectious Diseases
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- SARS CoV-2 antibody assays measure antibodies against the viral nucleoprotein (NP) or spike protein. The study examined if testing of antibodies against both antigens increases the diagnostic sensitivity. Sera (N=98) from infected individuals were tested with ELISAs based on the NP, receptor-binding domain (RBD), or both proteins. The AUROCs were 0.958 (NP), 0.991 (RBD), and 0.992 (NP/RBD). The RBD- and NP/RBD-based ELISAs showed better performance than the NP-based assay. Simultaneous testing for antibodies against NP and RBD increased the number of true and false positives. If maximum diagnostic sensitivity is required, the NP/RBD-based ELISA is preferable. Otherwise, the RBD-based ELISA is sufficient. Supplementary Information The online version contains supplementary material available at 10.1007/s10096-021-04284-5.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
2019-20 coronavirus outbreak
Coronavirus disease 2019 (COVID-19)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
SARS CoV-2
Enzyme-Linked Immunosorbent Assay
Antibodies, Viral
Antibodies
03 medical and health sciences
COVID-19 Testing
0302 clinical medicine
Protein Domains
Antigen
Humans
030212 general & internal medicine
Nucleoprotein
biology
SARS-CoV-2
Chemistry
Brief Report
COVID-19
Spike Protein
General Medicine
Virology
Receptor-binding domain
Nucleoproteins
030104 developmental biology
Infectious Diseases
biology.protein
ELISA
Antibody
Subjects
Details
- ISSN :
- 14354373 and 09349723
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- European Journal of Clinical Microbiology & Infectious Diseases
- Accession number :
- edsair.doi.dedup.....68f4a0c27b3af6cafb0cf4910055687f