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The Use of Xenosurveillance to Detect Human Bacteria, Parasites, and Viruses in Mosquito Bloodmeals
- Source :
- The American Journal of Tropical Medicine and Hygiene
- Publication Year :
- 2017
- Publisher :
- American Society of Tropical Medicine and Hygiene, 2017.
-
Abstract
- Infectious disease surveillance is hindered by several factors, including limited infrastructure and geographic isolation of many resource-poor regions. In addition, the complexities of sample acquisition, processing, and analysis, even in developed regions, can be rate limiting. Therefore, new strategies to survey human populations for emerging pathogens are necessary. Xenosurveillance is a method that utilizes mosquitoes as sampling devices to search for genetic signatures of pathogens in vertebrates. Previously we demonstrated that xenosurveillance can detect viral RNA in both laboratory and field settings. However, its ability to detect bacteria and parasites remains to be assessed. Accordingly, we fed Anopheles gambiae mosquitoes blood that contained Trypanosoma brucei gambiense and Bacillus anthracis. In addition, we determined whether two additional emerging viruses, Middle East Respiratory Syndrome Coronavirus and Zika virus could be detected by this method. Pathogen-specific real-time reverse transcription polymerase chain reaction was used to evaluate the sensitivity of xenosurveillance across multiple pathogen taxa and over time. We detected RNA from all pathogens at clinically relevant concentrations from mosquitoes processed up to 1 day postbloodfeeding. These results demonstrate that xenosurveillance may be used as a tool to expand surveillance for viral, parasitic, and bacterial pathogens in resource-limited areas.
- Subjects :
- 0301 basic medicine
Middle East respiratory syndrome coronavirus
Trypanosoma brucei gambiense
Anopheles gambiae
030231 tropical medicine
Antibodies, Protozoan
medicine.disease_cause
Zika virus
03 medical and health sciences
0302 clinical medicine
Virology
Anopheles
medicine
Animals
Humans
Pathogen
biology
Zika Virus Infection
Articles
biology.organism_classification
Antibodies, Bacterial
Bacillus anthracis
Reverse transcription polymerase chain reaction
030104 developmental biology
Infectious Diseases
Infectious disease (medical specialty)
Middle East Respiratory Syndrome Coronavirus
RNA, Viral
Parasitology
Bacteria
Subjects
Details
- ISSN :
- 14761645 and 00029637
- Volume :
- 97
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The American Journal of Tropical Medicine and Hygiene
- Accession number :
- edsair.doi.dedup.....68f32c85ba10e6ba7445215b9ec41c31
- Full Text :
- https://doi.org/10.4269/ajtmh.17-0063