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Control of Roof Plate Development and Signaling by Lmx1b in the Caudal Vertebrate CNS
- Source :
- The Journal of Neuroscience. 24:5694-5703
- Publication Year :
- 2004
- Publisher :
- Society for Neuroscience, 2004.
-
Abstract
- Numerous studies have identified the roof plate as an important signaling center controlling dorsal interneuron specification and differentiation in the developing spinal cord. Currently, the molecular pathways of roof plate formation and function are poorly understood. We determined that the LIM-homeodomain transcription factor Lmx1b is sufficient to induce functional roof plate in the early chick developing spinal cord. In the chick, Lmx1b acts upstream of Lmx1a in the roof plate developmental program. Once the roof plate forms, we show that Bmp and Wnt signaling are the major components ofLmx1a/b-dependent roof plate dorsal patterning activity. The roof plate function ofLmx1bis not conserved across vertebrates becauseLmx1bis not expressed in mouse roof plate progenitors. Instead, mouse caudal CNS roof plate formation relies entirely onLmx1a. Lmx1bcan, however, partially rescue roof plate development indreher(Lmx1a-/-) mice, indicating that Lmx1b has some functional redundancy to Lmx1a. Furthermore, we demonstrate that the roof plate-inducing activity of Lmx1b can be suppressed by Mash1 (Cash1), which is normally expressed in intermediate neural tube in both chick and mouse. Our data identifyLmx1bas a key regulator of spinal cord roof plate induction and function.
- Subjects :
- animal structures
Interneuron
Basal plate (neural tube)
Cellular differentiation
LIM-Homeodomain Proteins
Development/Plasticity/Repair
Chick Embryo
In Vitro Techniques
Biology
Mice
Interneurons
Basic Helix-Loop-Helix Transcription Factors
medicine
Animals
Homeodomain Proteins
General Neuroscience
Neural tube
Wnt signaling pathway
Cell Differentiation
Anatomy
Roof plate formation
Spinal cord
Cell biology
DNA-Binding Proteins
body regions
medicine.anatomical_structure
Spinal Cord
GDF7
embryonic structures
Transcription Factors
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....68d92f203d4f51fa9f42901cb90047dc