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Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients
- Source :
- Journal of Clinical Microbiology, Journal of Clinical Microbiology, American Society for Microbiology, 2016, 54 (6), pp.1641-1643. ⟨10.1128/JCM.03372-15⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- The impact of HIV-1 DNA coamplification during HIV-1 RNA quantification on dried blood spots (DBS) was explored. False-positive HIV RNA detection (22/62, 35%) was associated with high HIV-1 DNA levels. Specificity of HIV-1 RNA assays on DBS should be evaluated following manufacturer protocols on samples with HIV-1 DNA levels of ≥1,000 copies/10 6 peripheral blood mononuclear cells.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
030106 microbiology
HIV Infections
Biology
Peripheral blood mononuclear cell
Hiv 1 rna
Specimen Handling
03 medical and health sciences
chemistry.chemical_compound
Virology
Humans
False Positive Reactions
Desiccation
Diagnostic Errors
Dried blood
Hiv 1 dna
Spots
RNA
virus diseases
Viral Load
Molecular biology
3. Good health
030104 developmental biology
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
chemistry
DNA, Viral
HIV-1
RNA, Viral
Drug Monitoring
Viral load
DNA
Subjects
Details
- Language :
- English
- ISSN :
- 00951137
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology, Journal of Clinical Microbiology, American Society for Microbiology, 2016, 54 (6), pp.1641-1643. ⟨10.1128/JCM.03372-15⟩
- Accession number :
- edsair.doi.dedup.....68d7e2282d9ac8ac8ad1906a1401ae7b
- Full Text :
- https://doi.org/10.1128/JCM.03372-15⟩