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Thrown for a (stem) loop: How RNA structure impacts circular RNA regulation and function
- Source :
- Methods. 196:56-67
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Exonic circular RNAs (circRNAs) are RNA molecules that are covalently closed by back-splicing via canonical splicing machinery. Despite overlapping sequences, exon circularization generates RNA secondary structures through intramolecular base-pairing that are different from the linear transcript. Here we review factors that may affect circRNA structure and how structure affects circRNA function and regulation. We highlight considerations for RNA sequencing and expression measurement to ensure highly structured circRNAs are accurately represented by the data and discuss issues that need to be addressed in generating circRNAs to recapitulate their endogenous structures. We conclude our review by discussing experimental strategies on revealing the varied roles of RNA structure in circRNA biogenesis, function and decay.
- Subjects :
- 0303 health sciences
Base Sequence
RNA Splicing
030302 biochemistry & molecular biology
RNA
Exons
RNA, Circular
Computational biology
Biology
Stem-loop
General Biochemistry, Genetics and Molecular Biology
Nucleic acid secondary structure
03 medical and health sciences
Exon
Circular RNA
RNA splicing
Nucleic acid structure
Molecular Biology
Biogenesis
030304 developmental biology
Subjects
Details
- ISSN :
- 10462023
- Volume :
- 196
- Database :
- OpenAIRE
- Journal :
- Methods
- Accession number :
- edsair.doi.dedup.....68d2a90d4b93b12678350886e121bdf1