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Establishment of Induced Pluripotent Stem Cells from Centenarians for Neurodegenerative Disease Research
- Source :
- PLoS ONE, PLoS ONE, Vol 7, Iss 7, p e41572 (2012)
- Publication Year :
- 2012
- Publisher :
- Public Library of Science, 2012.
-
Abstract
- Induced pluripotent stem cell (iPSC) technology can be used to model human disorders, create cell-based models of human diseases, including neurodegenerative diseases, and in establishing therapeutic strategies. To detect subtle cellular abnormalities associated with common late-onset disease in iPSCs, valid control iPSCs derived from healthy donors free of serious late-onset diseases are necessary. Here, we report the generation of iPSCs from fibroblasts obtained immediately postmortem from centenarian donors (106- and 109-years-old) who were extremely healthy until an advanced age. The iPSCs were generated using a conventional method involving OCT4, SOX2, KLF4, and c-MYC, and then differentiated into neuronal cells using a neurosphere method. The expression of molecules that play critical roles in late-onset neurodegenerative diseases by neurons differentiated from the centenarian-iPSCs was compared to that of neurons differentiated from iPSCs derived from familial Alzheimer's disease and familial Parkinson's disease (PARK4: triplication of the α synuclein gene) patients. The results indicated that our series of iPSCs would be useful in neurodegeneration research. The iPSCs we describe, which were derived from donors with exceptional longevity who were presumed to have no serious disease risk factors, would be useful in longevity research and as valid super-controls for use in studies of various late-onset diseases.
- Subjects :
- Lewy Body Disease
Male
Science
Cellular differentiation
Induced Pluripotent Stem Cells
Disease
Biology
chemistry.chemical_compound
Kruppel-Like Factor 4
SOX2
Neural Stem Cells
Alzheimer Disease
Neurosphere
Neurobiology of Disease and Regeneration
medicine
Humans
Induced pluripotent stem cell
Cells, Cultured
Alpha-synuclein
Aged, 80 and over
Neurons
Multidisciplinary
Movement Disorders
Stem Cells
Neurodegeneration
Cell Differentiation
Neurochemistry
Parkinson Disease
medicine.disease
chemistry
Neurology
Immunology
alpha-Synuclein
Medicine
Dementia
Female
Alzheimer's disease
Molecular Neuroscience
Neuroscience
Research Article
Developmental Biology
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....68cbcfcd5151ca370d336fc626eb88d3