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Towards interference free HPLC-SERS for the trace analysis of drug metabolites in biological fluids
- Source :
- Journal of Pharmaceutical and Biomedical Analysis. 136:38-43
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Highlights - Sofosbuvir metabolite was studied for the first time by Raman spectroscopy. - Cheap and disposable paper substrate was utilized for the determination of PSI-6206 by HPLC-SERS. - The use of disposable substrate eliminated the memory effect problem in the HPLC-SERS. - The SERS LOQ of PSI-6206 was 13 ng L-1 (R2 = 0.959, RSD = 5.23%), 4 orders of magnitude less than HPLC-DAD. - The HPLC-SERS method provided unique structural identification of PSI-6206 similar to HPLC-MS Abstract Sofosbuvir metabolite, 2′-deoxy-2′-fluoro-2′-C-methyluridine (PSI-6206) was studied for the first time by surface enhanced Raman spectroscopy (SERS) using the paper-based SERS substrate. The quantification limit of PSI-6206 by SERS was found to be 13 ng L−1 (R2 value = 0.959, RSD = 5.23%). For the structural and quantitative analysis of PSI-6206 in blood plasma, an interference-free HPLC-SERS method was developed and compared to HPLC-DAD and HPLC–MS methods. The SERS quantification of the drug by the paper substrate was 4 orders of magnitude more sensitive than that by the diode array detector. In addition, the SERS detection provided unique structural identification of the drug in blood plasma, similar to Mass spectroscopy detector. Due to the disposable nature of the SERS substrate, the new method does not suffer from the known “memory effect” which is known to lead to false positive identification in traditional HPLC-SERS methods. Therefore, the presented HPLC-paper SERS platform holds great potential for the sensitive and cost effective determination of drugs and their metabolites in biological fluids.
- Subjects :
- Metabolite
Clinical Biochemistry
Analytical chemistry
Pharmaceutical Science
02 engineering and technology
In Vitro Techniques
Spectrum Analysis, Raman
Mass spectrometry
Antiviral Agents
01 natural sciences
Analytical Chemistry
chemistry.chemical_compound
symbols.namesake
Limit of Detection
Chromatography detector
Drug Discovery
Animals
Horses
Chromatography, High Pressure Liquid
Spectroscopy
010401 analytical chemistry
Substrate (chemistry)
Reference Standards
Surface-enhanced Raman spectroscopy
021001 nanoscience & nanotechnology
Deoxyuridine
Orders of magnitude (mass)
Nanostructures
0104 chemical sciences
chemistry
symbols
Gold
Sofosbuvir
0210 nano-technology
Raman spectroscopy
Quantitative analysis (chemistry)
Subjects
Details
- ISSN :
- 07317085
- Volume :
- 136
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmaceutical and Biomedical Analysis
- Accession number :
- edsair.doi.dedup.....68a1e32966128b31f1de28f92ca33a9a