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A method for high-throughput image-based antifungal screening
- Source :
- Journal of microbiological methods. 190
- Publication Year :
- 2021
-
Abstract
- Robust antifungal screening is technically challenging particularly for filamentous fungi. We present a method for undertaking antifungal screening assays that builds upon existing broth dilution protocols and incorporates time resolved image-based assessment of fungal growth. We show that the method performs with different fungi, particularly those for which spores can be used as inoculum, and with different compound classes, can accurately assess susceptibility or otherwise in only few hours and can even account for differences in inherent growth properties of strains.
- Subjects :
- Microbiology (medical)
Antifungal
Fusarium
Aspergillus
Antifungal Agents
biology
medicine.drug_class
fungi
Broth dilution
Drug Evaluation, Preclinical
Fungi
food and beverages
Microbial Sensitivity Tests
Verticillium
biology.organism_classification
Microbiology
Spore
High-Throughput Screening Assays
medicine
Image Processing, Computer-Assisted
Food science
Molecular Biology
Throughput (business)
Image based
Subjects
Details
- ISSN :
- 18728359
- Volume :
- 190
- Database :
- OpenAIRE
- Journal :
- Journal of microbiological methods
- Accession number :
- edsair.doi.dedup.....688da158161ee2121b6549bc69682114